State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
J Biomed Mater Res B Appl Biomater. 2011 Apr;97(1):74-83. doi: 10.1002/jbm.b.31788. Epub 2011 Feb 2.
Nano-hydroxyapatite (n-HA)/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL, PCEC) composite membranes were prepared by solvent casting and evaporation method. The structure and properties of the membranes were investigated by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), water contact angle measurements, in vitro hydrolytic degradation, mechanical test, and cell culture. The effect of n-HA content on physical-chemical properties of the n-HA/PCEC composite membranes was studied. The results showed that the shape and size of micropores of the composite membranes changed with n-HA content increased; the tensile strength decreased with the increase of n-HA content. The osteoblast cell was cultured on the membranes, good cell attachment and growth manner were observed after postseeding for 1 day. MTT assays showed that the n-HA/PCEC membranes had no negative effect on the cell viability and proliferation. These results suggested that the obtained n-HA/PCEC composite membranes in this study might have prospective applications in tissue engineering field.
纳米羟基磷灰石（n-HA）/聚（ε-己内酯）-聚乙二醇-聚（ε-己内酯）（PCL-PEG-PCL，PCEC）复合膜通过溶剂浇铸和蒸发方法制备。通过傅里叶变换红外光谱（FT-IR）、X 射线衍射（XRD）、扫描电子显微镜（SEM）、水接触角测量、体外水解降解、力学试验和细胞培养来研究膜的结构和性能。研究了 n-HA 含量对 n-HA/PCEC 复合膜物理化学性质的影响。结果表明，随着 n-HA 含量的增加，复合膜的微孔形状和尺寸发生变化；拉伸强度随 n-HA 含量的增加而降低。将成骨细胞接种在膜上，接种后 1 天观察到良好的细胞附着和生长方式。MTT 分析表明，n-HA/PCEC 膜对细胞活力和增殖没有负面影响。这些结果表明，本研究中获得的 n-HA/PCEC 复合膜可能在组织工程领域具有广阔的应用前景。
