Department of Civil and Environmental Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.
Laboratory of Environmental Microbiology and Toxicology, School of Biological Sciences, Faculty of Science, The University of Hong Kong, Pokfulam Road, Pok Fu Lam, Hong Kong, China.
Appl Microbiol Biotechnol. 2019 Feb;103(4):1953-1960. doi: 10.1007/s00253-018-9521-4. Epub 2019 Jan 2.
Anaerobic ammonium oxidation (anammox) has been widely applied for biological nitrogen removal in freshwater systems, and there is a potential for its extension in saline water systems. In this study, the abundance and biodiversity of anammox bacteria were investigated in both saline and freshwater full-scale sewage treatment plants (STPs). The anammox bacteria were widely found in four tested STPs with abundance of 10-10 copies per mL of 16S rRNA gene. Phylogenetic results showed that Ca. Scalindua and Ca. Brocadia dominated in saline and freshwater STPs, respectively. Ca. Kuenenia dominated in one of freshwater STPs. However, redundancy discriminate analysis (RDA) indicates the distribution of Ca. Kuenenia in both saline and freshwater conditions. To further elucidate these observations, the Monod model was integrated with Gauss equation for the evaluation of salinity-induced kinetics. Model results reveal that when nitrite concentration (S) is higher than nitrite affinity constant (K), salinity (over ~ 3.0%) is responsible for Candidatus Scalindua dominance over Candidatus Kuenenia. Conversely, in nitrite-depleted conditions (K ≥ S), high nitrite affinity leads to the predominance of Ca. Scalindua in all salinities. This study provides fundamental insights into saline anammox applications.
厌氧氨氧化（anammox）已广泛应用于淡水系统中的生物脱氮，并且在咸水系统中也有扩展的潜力。本研究调查了咸水和淡水产污水处理厂（STP）中厌氧氨氧化菌的丰度和生物多样性。在四个测试的 STP 中均广泛发现了厌氧氨氧化菌，其 16S rRNA 基因的丰度为 10-10 拷贝/毫升。系统发育结果表明，Ca. Scalindua 和 Ca. Brocadia 分别在咸水和淡水 STP 中占优势。Ca. Kuenenia 在一个淡水 STP 中占优势。然而，冗余判别分析（RDA）表明 Ca. Kuenenia 在咸水和淡水条件下的分布。为了进一步阐明这些观察结果，将 Monod 模型与 Gauss 方程集成，以评估盐度诱导动力学。模型结果表明，当亚硝酸盐浓度（S）高于亚硝酸盐亲和力常数（K）时，盐度（超过约 3.0%）导致 Candidatus Scalindua 对 Candidatus Kuenenia 的优势。相反，在亚硝酸盐耗尽的条件下（K≥S），高亚硝酸盐亲和力导致 Ca. Scalindua 在所有盐度下占优势。本研究为咸水厌氧氨氧化应用提供了基础见解。
