Ji Xiaoming, Wang Yongli, Lee Po-Heng
Department of Civil and Environmental Engineering, Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.
Department of Civil and Environmental Engineering, Imperial College London, South Kensington Campus, London, UK.
Appl Microbiol Biotechnol. 2020 Jun;104(12):5593-5604. doi: 10.1007/s00253-020-10598-9. Epub 2020 Apr 17.
The salinity effect on anammox bacteria has been widely reported; however, rare studies describe the microbial dynamics of anammox-based process response to the introduction of real seawater at mainstream conditions. In this study, an anammox process at mainstream conditions without pre-enriching anammox bacteria was shifted to the feeds of a synthetic wastewater with a portion of seawater mixture. It achieved over 0.180 kg-N/(m day) of nitrogen removal rate with an additional seawater proportion of 20% in the influent. The bacterial biodiversity was significantly increased with the increase of seawater proportions. High relative abundance of anammox bacteria (34.24-39.92%) related to Ca. Brocadia was enriched and acclimated to the saline environment. However, the introduction of seawater caused the enrichment of nitrite-oxidizing Ca. Nitrospira, which was responsible for the deterioration of nitrogen removal efficiency. Possible adaptation metabolisms in anammox bacteria and other nitrogen transforming bacteria are discussed. These results highlight the importance of microbial diversity for anammox process under the saline environments of 20% and 40% seawater composition.
盐度对厌氧氨氧化细菌的影响已有广泛报道；然而，很少有研究描述在主流条件下引入实际海水时基于厌氧氨氧化的工艺响应的微生物动态。在本研究中，在没有预先富集厌氧氨氧化细菌的主流条件下，将一个厌氧氨氧化工艺切换至含有一部分海水混合物的合成废水进料。在进水额外海水比例为20%的情况下，其实现了超过0.180 kg-N/(m³·天)的氮去除率。随着海水比例的增加，细菌生物多样性显著增加。与“Ca. Brocadia”相关的厌氧氨氧化细菌的相对丰度较高（34.24 - 39.92%），并被富集且适应了含盐环境。然而，海水的引入导致了负责氮去除效率恶化的亚硝酸盐氧化菌“Ca. Nitrospira”的富集。讨论了厌氧氨氧化细菌和其他氮转化细菌中可能的适应代谢。这些结果突出了在海水组成比例为20%和40%的含盐环境下微生物多样性对厌氧氨氧化工艺的重要性。
