马中miR-221-3p及其靶基因的表达分析
Expression analysis of miR-221-3p and its target genes in horses.
作者信息
Kim So-Won, Jo Ara, Im Jennifer, Lee Hee-Eun, Kim Heui-Soo
机构信息
Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan, 46241, Republic of Korea.
Institute of Systems Biology, Pusan National University, Busan, 46241, Republic of Korea.
出版信息
Genes Genomics. 2019 Apr;41(4):459-465. doi: 10.1007/s13258-018-00778-3. Epub 2019 Jan 2.
BACKGROUND
A microRNA (miRNA) is a small non-coding RNA (ncRNA) approximately 20 nucleotides long and it affects gene expression through mRNA cleavage or translational repression. Horses (Equus caballus) have been domesticated and bred to enhance their speed for racing. It has been studied extensively with genetic diversity, origins and evolution.
OBJECTIVES
We examined expression patterns of miR-221-3p and its target gene CDKN1C in various horse tissues.
METHODS
We used bioinformatic tools to examine target gene, seed region and evolutionary conservation of miR-221-3p. The expression patterns of miR-221-3p and its target gene CDKN1C were analyzed by quantitative polymerase chain reaction (qPCR).
RESULTS
Among eight tissues of horse, miR-221-3p was highly expressed in cerebellum and spleen. On the other hand, only medulla was highly expressed in CDKN1C gene.
CONCLUSION
Our study provides expression data of miR-221-3p and CDKN1C gene in horse and suggests the fundamental information for future studies in relation to functional importance.
背景
微小RNA（miRNA）是一种长度约为20个核苷酸的小型非编码RNA（ncRNA），它通过mRNA切割或翻译抑制来影响基因表达。马（Equus caballus）已被驯化和培育以提高其赛跑速度。人们对其遗传多样性、起源和进化进行了广泛研究。
目的
我们研究了miR-221-3p及其靶基因CDKN1C在马的各种组织中的表达模式。
方法
我们使用生物信息学工具来研究miR-221-3p的靶基因、种子区域和进化保守性。通过定量聚合酶链反应（qPCR）分析miR-221-3p及其靶基因CDKN1C的表达模式。
结果
在马的八个组织中，miR-221-3p在小脑和脾脏中高表达。另一方面，只有延髓中CDKN1C基因高表达。
结论
我们的研究提供了马中miR-221-3p和CDKN1C基因的表达数据，并为未来有关功能重要性的研究提供了基础信息。
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