酿酒酵母中硫胺素转运的不可逆性。

The irreversibility of thiamin transport in Saccharomyces cerevisiae.

作者信息

Ruml T, Silhánková L, Rauch P

机构信息

Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague.

出版信息

Folia Microbiol (Praha). 1988;33(5):372-6. doi: 10.1007/BF02925847.

DOI:10.1007/BF02925847

PMID:3060416

Abstract

Neither exit nor counterflow efflux of thiamin, taken up previously by an active transport, were found in Saccharomyces cerevisiae, in either the wild type or a mutant with a lower rate of thiamin phosphorylation. Complete inhibition of thiamin phosphorylation by oxythiamin did not lead to any release of thiamin taken up by the cell.

摘要

在酿酒酵母中，无论是野生型还是硫胺素磷酸化速率较低的突变体，均未发现先前通过主动转运摄取的硫胺素存在外向或逆流流出的情况。氧硫胺素对硫胺素磷酸化的完全抑制并未导致细胞摄取的硫胺素释放。