Laboratorio de Biocatálisis y Biotransformaciones, Departamento de Química Orgánica y, Departamento de Biociencias, Facultad de Química, Universidad de la República (UdelaR), CP 11800, Montevideo, Uruguay.
LaBioChem, Institute of Chemistry, University of Campinas, Campinas, SP, 13084-971, Brazil.
World J Microbiol Biotechnol. 2019 Jan 2;35(1):12. doi: 10.1007/s11274-018-2586-5.
Microorganisms were isolated from industrial wool scouring effluents and from the soil adjacent to the wastewater treatment lagoon, both sterols-rich environments, in order to search for novel biocatalysts able to transform cholesterol. The isolates were identified on the basis of morphological and biochemical characteristics and phylogenetic analysis. Furthermore, a rapid and accurate bacteria identification by matrix assisted laser desorption/ionization-time-of-flight mass spectrometry was carried out. Bacteria and fungi including representatives of the genera Fusarium, Talaromyces, Trichoderma, Mucor, Aspergillus, Citrobacter, Proteus, Klebsiella, Exiguobacterium, Acinetobacter, Tsukamurella, Bacillus, and Streptomyces were found and evaluated for their ability to biotransform cholesterol by whole-cell treatment system. The results show that a Trichoderma koningiopsis strain, as well as two strains of Mucor circinelloides were able to transform cholesterol into value-added products. The major products were characterized as 7β-hydroxycholesterol, 4-cholesten-3-one, 5α,6α-epoxycholestan-3β-ol and 5β,6β-epoxycholestan-3β-ol. To the best of our knowledge, the present study is the first report of cholesterol biotransformation by representatives of Trichoderma and Mucor genera.
从富含甾醇的工业羊毛洗毛废水和废水处理池附近的土壤中分离到微生物，以寻找能够转化胆固醇的新型生物催化剂。根据形态学和生化特征以及系统发育分析对分离物进行了鉴定。此外，还通过基质辅助激光解吸/电离-飞行时间质谱进行了快速准确的细菌鉴定。发现了细菌和真菌，包括镰刀菌属、拟青霉属、木霉属、毛霉属、曲霉属、柠檬酸杆菌属、变形杆菌属、克雷伯菌属、极端杆菌属、不动杆菌属、土生梭菌属、芽孢杆菌属和链霉菌属的代表种，并通过全细胞处理系统评估了它们转化胆固醇的能力。结果表明，康宁木霉和两株毛霉能够将胆固醇转化为有价值的产物。主要产物被鉴定为 7β-羟基胆固醇、4-胆甾烯-3-酮、5α,6α-环氧胆甾烷-3β-醇和 5β,6β-环氧胆甾烷-3β-醇。据我们所知，本研究首次报道了木霉属和毛霉属代表种对胆固醇的生物转化。
