一种由WHX1301对胆固醇进行生物转化产生的黄嘌呤氧化酶抑制活性成分。
A xanthine oxidase inhibit activity component from biotransformation of cholesterol by WHX1301.
作者信息
Wei Haixin, Yang Chiguang, Weng Chenhong, Zhu Xiangdong
机构信息
College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, 330045, China.
出版信息
Heliyon. 2023 Feb 28;9(3):e14160. doi: 10.1016/j.heliyon.2023.e14160. eCollection 2023 Mar.
Steroids are one of the most widely used groups of medicines presently. There are some steroid drugs that have acquired with the transformation of microorganism. It's indispensability to screen the strain that is able to utilize steroids to generate new products. This study has screened a transformation strain WHX1301 that have ability to convert cholesterol. Based on the 16S rRNA gene sequence comparison, the isolate WHX1301 has been demonstrated to most similar as . Separation and purification of transformation product were identifying by NMR and ESI-MS. The major of product was 2,7-dihydroxycholesterol, and the by-product were 7-Hydroxycholestane-3,5-diene, Cholesterane-3,5-diene. Fortunately, 2,7-dihydroxycholesterol has inhibitory activity against xanthine oxidase with a 34.8% inhibition rate at a concentration of 20 μg/ml. Using the resting cells of WHX1301 to transform cholesterol, the product yield can reach 76%. Present paper is the first report regarding the microbial transformation of steroids by .
类固醇是目前使用最广泛的药物类别之一。有一些类固醇药物是通过微生物转化获得的。筛选能够利用类固醇产生新产品的菌株是必不可少的。本研究筛选出了一株具有转化胆固醇能力的转化菌株WHX1301。基于16S rRNA基因序列比较，已证明分离株WHX1301与……最为相似。通过核磁共振（NMR）和电喷雾电离质谱（ESI-MS）对转化产物进行分离和纯化鉴定。主要产物为2,7-二羟基胆固醇，副产物为7-羟基胆甾-3,5-二烯、胆甾-3,5-二烯。幸运的是，2,7-二羟基胆固醇对黄嘌呤氧化酶具有抑制活性，在浓度为20μg/ml时抑制率为34.8%。使用WHX1301的静息细胞转化胆固醇，产物产率可达76%。本文是关于……对类固醇进行微生物转化的首次报道。
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