Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo, SP, Brazil.
Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, SP, Brazil.
Photodiagnosis Photodyn Ther. 2021 Sep;35:102464. doi: 10.1016/j.pdpdt.2021.102464. Epub 2021 Jul 25.
The aim of this study was to evaluate curcumin-mediated antimicrobial photodynamic therapy (aPDT) action combined or not with ethylenediaminetetraacetic acid (EDTA) and hydroxyethylidene bisphosphonate (HEBP) on Enterococcus faecalis biofilms.
Enterococcus faecalis biofilms were grown on dentin bovine discs in brain heart infusion (BHI) medium with 1% glucose, in aerobic conditions at 37°C for 7 days. Then, they were randomly distributed to one of experimental conditions, as follows: control, 75 J.cm LED, 600 μmol.L curcumin, 17% EDTA, 18% HEBP, 600 μmol.L curcumin plus 75 J.cm LED, 600 μmol.L curcumin plus 17% EDTA, 600 μmol.L curcumin plus 18% HEBP, 600 μmol.L curcumin plus 17% EDTA and 75 J.cm LED or 600 μmol.L curcumin plus 18% HEBP and 75 J.cm LED. The viability of microorganisms and the vitality of biofilms were determined by colony forming unit counts and confocal scanning laser microscopy (CSLM), respectively. Statistical analysis was conducted by Kruskal Wallis and Dunn's post-hoc tests (α = 0.05).
The results showed that all combinations of aPDT with chelators significantly reduced the viability of microbial cells and the vitality of biofilms in comparison to control, even when considering deeper layers of biofilms.
The combination of curcumin with EDTA and HEBP similarly improved the effect of aPDT on E. faecalis biofilms.
本研究旨在评估姜黄素介导的光动力抗菌疗法（aPDT）与乙二胺四乙酸（EDTA）和羟乙基二膦酸（HEBP）联合应用对粪肠球菌生物膜的作用。
粪肠球菌生物膜在含 1%葡萄糖的脑心浸液（BHI）培养基中于牛牙本质圆盘上，在 37°C 有氧条件下培养 7 天。然后，将它们随机分配到以下实验条件之一：对照组、75 J.cm LED、600 μmol.L 姜黄素、17% EDTA、18% HEBP、600 μmol.L 姜黄素加 75 J.cm LED、600 μmol.L 姜黄素加 17% EDTA、600 μmol.L 姜黄素加 18% HEBP、600 μmol.L 姜黄素加 17% EDTA 和 75 J.cm LED 或 600 μmol.L 姜黄素加 18% HEBP 和 75 J.cm LED。通过平板计数法和共聚焦扫描激光显微镜（CSLM）分别测定微生物的存活率和生物膜的活力。采用 Kruskal Wallis 和 Dunn 事后检验（α=0.05）进行统计学分析。
结果表明，与对照组相比，aPDT 与螯合剂的所有组合均显著降低了微生物细胞的存活率和生物膜的活力，即使考虑到生物膜的更深层。
姜黄素与 EDTA 和 HEBP 的联合应用同样提高了 aPDT 对粪肠球菌生物膜的作用。
