Arbouche Nadia, Raul Jean-Sebastien, Garnier Delphine, Kintz Pascal, Ameline Alice
Institut de médecine légale, Strasbourg, France.
Faculté de Pharmacie, Illkirch, France.
Drug Test Anal. 2019 Apr;11(4):610-616. doi: 10.1002/dta.2564. Epub 2019 Jan 10.
Synthetic cannabinoid receptor agonists were first identified in herbal products in 2008 advertised as a legal replacement for cannabis. These herbal incense are usually called "spice" and among these, one product in particular has gained popularity: AB-PINACA (N-[(2S)-1-Amino-3-methyl-1-oxobutan-2-yl]-1-pentyl-1H-indazole-3-carboxamide). This drug has been discovered to have a stronger binding to human cannabinoid CB and CB receptors than ∆ -THC.While some articles have been published regarding the presence of AB-PINACA in biological fluids such as blood and urine, none reports the presence of AB-PINACA in hair. We have developed and validated a method for detection of AB-PINACA in hair using a liquid chromatography-tandem mass spectrometry system and applied it to head and pubic hair obtained in a case of intoxication. The validation procedure demonstrated a limit of detection and a limit of quantification of 0.5 and 1 pg/mg, respectively and acceptable linearity, repeatability, and reproducibility. AB-PINACA tested positive in the blood (5.7 ng/mL) and less than 1 ng/mL was found in urine. The analysis of the hair specimens resulted in an unusual distribution of the drug between head and pubic hair. AB-PINACA was identified at a higher concentration in head hair (195 pg/mg) versus in pubic hair (5 pg/mg). The very low concentration of AB-PINACA in the urine after consumption, due to rapid metabolism, could explain this infrequent distribution, as pubic hair can be contaminated by urine. In any case, it cannot be excluded that the high concentration in head hair may be due to environmental contamination.
合成大麻素受体激动剂于2008年首次在草药产品中被发现，这些产品被宣传为大麻的合法替代品。这些草药香薰通常被称为“香料”，其中一种产品尤其受欢迎：AB-PINACA（N-[(2S)-1-氨基-3-甲基-1-氧代丁烷-2-基]-1-戊基-1H-吲唑-3-甲酰胺）。已发现这种药物与人大麻素CB1和CB2受体的结合力比Δ9-四氢大麻酚更强。虽然已经发表了一些关于AB-PINACA在血液和尿液等生物流体中存在情况的文章，但没有一篇报道AB-PINACA在毛发中的存在情况。我们开发并验证了一种使用液相色谱-串联质谱系统检测毛发中AB-PINACA的方法，并将其应用于一例中毒案件中获取的头发和阴毛样本。验证程序表明检测限和定量限分别为0.5和1 pg/mg，并且具有可接受的线性、重复性和再现性。AB-PINACA在血液中检测呈阳性（5.7 ng/mL），尿液中检测到的含量低于1 ng/mL。对毛发样本的分析结果显示，该药物在头发和阴毛中的分布情况异常。AB-PINACA在头发中的浓度（195 pg/mg）高于阴毛中的浓度（5 pg/mg）。由于代谢迅速，食用后尿液中AB-PINACA的浓度非常低，这可能解释了这种不常见的分布情况，因为阴毛可能会被尿液污染。无论如何，不能排除头发中高浓度的AB-PINACA可能是由于环境污染所致。
