IEEE Trans Biomed Circuits Syst. 2018 Dec;12(6):1440-1449. doi: 10.1109/TBCAS.2018.2870297.
This paper presents a hand-held point-of-care device that incorporates a lab-on-a-chip module with interdigitated capacitive biosensors for label-free detection of multiple cancer and cardiovascular disease biomarkers. The developed prototype is comprised of a cartridge incorporating capacitive biodetection sensors, a sensitive capacitive readout electronics enclosed in a hand-held unit, and data analysis software calculating the concentration of biomarkers using previously stored reference database. The capacitive biodetection sensors are made of interdigitated circular electrodes, which are preactivated with single (for detecting one biomarker) or multiple specific antibodies (for detecting multiple disease biomarkers). Detection principle of capacitive biosensor is based on measuring the level of capacitance change between interdigitated electrode pairs induced by the change in dielectric constant due to affinity-based electron exchange in between antibodies/antigens and electrodes. The more antibody-antigens binding occurs, the more capacitance change is measured due to the change in dielectric constant of the capacitance media. The device uses preactivated ready-to-use cartridges embedded with capacitive biosensors with shelf-life of three months under optimal conditions, and is capable of onsite diagnosis and can report the result in less than 30 min. The device is verified with real patient blood samples for six different disease biomarkers.
本文提出了一种手持式即时检测设备，它将芯片实验室模块与叉指式电容生物传感器结合在一起，用于无标记检测多种癌症和心血管疾病生物标志物。所开发的原型由一个包含电容生物检测传感器的盒式组件、一个包含在手持式单元内的敏感电容读出电子设备，以及使用先前存储的参考数据库计算生物标志物浓度的数据分析软件组成。电容生物检测传感器由叉指式圆形电极制成，这些电极预先用单个（用于检测一种生物标志物）或多个特定抗体（用于检测多种疾病生物标志物）进行激活。电容生物传感器的检测原理基于测量由于抗体/抗原和电极之间基于亲和力的电子交换导致介电常数变化而引起的叉指电极对之间电容变化的水平。由于电容介质的介电常数发生变化，抗体-抗原结合越多，测量到的电容变化就越大。该设备使用预先激活的即用型盒式组件，其中嵌入了带有电容生物传感器的即用型盒式组件，在最佳条件下保质期为三个月，能够进行现场诊断，并在不到 30 分钟内报告结果。该设备已通过 6 种不同疾病生物标志物的真实患者血液样本进行了验证。
