Aquatic Biology Laboratory, Department of Zoology, Panjab University, Chandigarh, India.
Aquatic Biology Laboratory, Department of Zoology, Panjab University, Chandigarh, India.
Environ Toxicol Pharmacol. 2019 Feb;66:62-68. doi: 10.1016/j.etap.2018.12.014. Epub 2018 Dec 21.
With the known facts of deltamethrin toxicity in aquaculture, efforts have been made to ameliorate its toxicity with supplementation of Silybum marianum plant extract in Cyprinus carpio. For validating its efficacy, standard techniques of histopathology, anti-oxidant profile and RAPD-PCR were used. By performing acute toxicity bioassay, 96 h LC of the pesticide to C. carpio was determined and was found to be 2 μg/L. Histopathological alterations were comprised of nuclear alterations, vacuolisation and oedema in the hepatocytes. Chronic exposure to the toxicant induced significant changes in antioxidant defense system (CAT, SOD, GSH and GST levels), lipid peroxidation being prominent. Diet supplementation with silymarin appeared to modulate the oxidative stress, histopathological alterations and genotoxic damage caused by the pesticide in the fish. RAPD-PCR studies revealed deltamethrin induced toxicity and its effective amelioration in form of restoration of bands which were lost in toxicant exposed DNA profile.
鉴于二氯苯醚菊酯在水产养殖中的毒性已被广泛认知，人们已尝试通过添加水飞蓟素植物提取物来减轻其毒性，这一方法已在鲤鱼身上得到验证。本研究采用组织病理学、抗氧化谱和 RAPD-PCR 等标准技术来验证其功效。通过进行急性毒性生物测定，确定了该农药对鲤鱼的 96 小时半数致死浓度（LC），结果为 2μg/L。组织病理学变化包括肝细胞的核改变、空泡化和水肿。长期暴露于该毒物会导致抗氧化防御系统（CAT、SOD、GSH 和 GST 水平）发生显著变化，脂质过氧化作用尤为明显。饲料中添加水飞蓟素似乎可以调节鲤鱼体内由农药引起的氧化应激、组织病理学改变和遗传毒性损伤。RAPD-PCR 研究表明，二氯苯醚菊酯会引起毒性，而水飞蓟素的添加则可以有效减轻这种毒性，表现为在毒物暴露的 DNA 图谱中丢失的条带得到恢复。
