School of Physical Science and Technology, ShanghaiTech University, 393 Middle Huaxia Road, Pudong, Shanghai 201210, China; Shanghai Institute of Organic Chemistry, Chinese Academy of Science, 345 Lingling Road, Shanghai 200032, China.
School of Physical Science and Technology, ShanghaiTech University, 393 Middle Huaxia Road, Pudong, Shanghai 201210, China.
Mater Sci Eng C Mater Biol Appl. 2019 Mar;96:616-624. doi: 10.1016/j.msec.2018.11.072. Epub 2018 Nov 29.
Intermediate filaments, together with actin microfilaments and microtubules constituent the cytoskeleton of mammalian cells, involving in various cellular activities. The roles of intermediate filaments in cell skeleton reorganization when responding with extracellular matrix (ECM) nanostructure are poorly understood yet. To unveil the effects of fibrous composition and orientation on cells, we developed electrospun nanofibers of varying topology and components, and the effects on assembly of intermediate filaments as keratin and vimentin were investigated in detail. We found that aligned nanofibers enhanced expression of E-cadherin and promoted assembly of keratin intermediate filaments. Meanwhile, the compositional variation show different preference on up-regulation of the two intermediate filaments. Compared to keratin, the assembly of vimentin intermediate filaments were promoted by incorporating bovine serum albumin (BSA) functionalized graphene oxide (BSA-GO) into polycaprolactone (PCL) nanofibers. Thus, our findings elucidate how the different physical factors of fibrous extracellular matrix affect the reorganization of cytoskeleton by assembly of keratin and vimentin intermediate filaments.
中间丝与肌动蛋白微丝和微管一起构成哺乳动物细胞的细胞骨架，参与各种细胞活动。然而，中间丝在响应细胞外基质（ECM）纳米结构时在细胞骨架重排中的作用还不清楚。为了揭示纤维组成和取向对细胞的影响，我们开发了具有不同拓扑结构和组成的静电纺纳米纤维，并详细研究了它们对角蛋白和波形蛋白中间丝组装的影响。我们发现，取向纳米纤维增强了 E-钙黏蛋白的表达，并促进了角蛋白中间丝的组装。同时，组成变化对两种中间丝的上调表现出不同的偏好。与角蛋白相比，将牛血清白蛋白（BSA）功能化氧化石墨烯（BSA-GO）掺入聚己内酯（PCL）纳米纤维中会促进波形蛋白中间丝的组装。因此，我们的研究结果阐明了纤维细胞外基质的不同物理因素如何通过角蛋白和波形蛋白中间丝的组装来影响细胞骨架的重排。
