The State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, Zhejiang University, Hangzhou 310028, China.
Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School of Mechanical Engineering, Zhejiang University, Hangzhou 310028, China.
Biosci Rep. 2019 Jan 18;39(1). doi: 10.1042/BSR20181748. Print 2019 Jan 31.
Recent studies have shown the importance of cell-substrate interaction on neurone outgrowth, where the Young's modulus of the matrix plays a crucial role on the neurite length, migration, proliferation, and morphology of neurones. In the present study, PC12 cells were selected as the representative neurone to be cultured on hydrogel substrates with different stiffness to explore the effect of substrate stiffness on the neurone outgrowth. By adjusting the concentration of gelatin methacryloyl (GelMA), the hydrogel substrates with the variation of stiffnesses (indicated by Young's modulus) from approximately 3-180 KPa were prepared. It is found that the stiffness of GelMA substrates influences neuronal outgrowth, including cell viability, adhesion, spreading, and average neurite length. Our results show a critical range of substrate's Young's modulus that support PC12 outgrowth, and modulate the cell characteristics and morphology. The present study provides an insight into the relationship between the stiffness of GelMA hydrogel substrates and PC12 cell outgrowth, and helps the design and optimization of tissue engineering scaffolds for nerve regeneration.
最近的研究表明细胞-基质相互作用对神经元生长的重要性，其中基质的杨氏模量对轴突长度、迁移、增殖和神经元形态有至关重要的影响。在本研究中，选择 PC12 细胞作为代表性神经元，在具有不同硬度的水凝胶基质上进行培养，以探索基质硬度对神经元生长的影响。通过调整明胶甲基丙烯酰（GelMA）的浓度，可以制备杨氏模量从约 3-180 KPa 变化的水凝胶基质。结果表明，GelMA 基质的硬度影响神经元的生长，包括细胞活力、黏附、铺展和平均轴突长度。我们的结果表明，有一个支持 PC12 生长的基质杨氏模量的关键范围，并调节细胞特性和形态。本研究深入了解了 GelMA 水凝胶基质的硬度与 PC12 细胞生长之间的关系，有助于神经再生组织工程支架的设计和优化。
