伊朗马尔卡齐省土壤样本中棘阿米巴的分离与基因分型
Isolation and Genotyping of Acanthamoeba from Soil Samples in Markazi Province, Iran.
作者信息
Meighani Mehri, Eslamirad Zahra, Hajihossein Reza, Ahmadi Azam, Saki Sassan
机构信息
Department of Biology, School of Basic Sciences, Islamic Azad University, Arak Branch, Arak, Iran.
Molecular and Medicine Research Center, Department of Medical Parasitology and Mycology, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
出版信息
Open Access Maced J Med Sci. 2018 Dec 16;6(12):2290-2294. doi: 10.3889/oamjms.2018.454. eCollection 2018 Dec 20.
AIM
A previous study confirmed the contamination of water sources with this parasite in Arak, Markazi Province, Iran. The current study investigated soil sources and determined the predominant genotype of Acanthamoeba in this region of Iran.
MATERIAL AND METHODS
Forty-eight soil samples, collected from different regions of Arak, Markazi province, Iran, were evaluated in this study. The samples were processed and identified by culturing on a specific medium, performing PCR assay, and sequencing the PCR products. Finally, using the NCBI database, the genotypes were determined.
RESULTS
Of 48 soil samples, 33.3% and 31.25% were contaminated with Acanthamoeba according to the culture and molecular assays, respectively. The majority of these isolates belonged to the T4, T5 and T6 genotypes of Acanthamoeba.
CONCLUSION
The genotypes of most isolates from soil samples in Arak similar to other regions of Iran belong to T4 genotype of this parasite. New sequence accession numbers include MG066681 and MG298785-MG298794.
目的
先前的一项研究证实了伊朗马尔卡齐省阿拉克的水源受到这种寄生虫的污染。本研究调查了土壤来源，并确定了伊朗该地区棘阿米巴的主要基因型。
材料与方法
本研究对从伊朗马尔卡齐省阿拉克不同地区采集的48份土壤样本进行了评估。通过在特定培养基上培养、进行PCR检测以及对PCR产物进行测序来处理和鉴定样本。最后，利用NCBI数据库确定基因型。
结果
根据培养和分子检测，48份土壤样本中分别有33.3%和31.25%被棘阿米巴污染。这些分离株大多数属于棘阿米巴的T4、T5和T6基因型。
结论
阿拉克土壤样本中大多数分离株的基因型与伊朗其他地区相似，属于该寄生虫的T4基因型。新的序列登录号包括MG066681和MG298785 - MG298794。
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