Bayat Hadi, Naderi Fatemeh, Khan Amjad Hayat, Memarnejadian Arash, Rahimpour Azam
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Adv Pharm Bull. 2018 Nov;8(4):591-597. doi: 10.15171/apb.2018.067. Epub 2018 Nov 29.
Clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein nuclease (Cas) is identified as an adaptive immune system in archaea and bacteria. Type II of this system, CRISPR-Cas9, is the most versatile form that has enabled facile and efficient targeted genome editing. Viral infections have serious impacts on global health and conventional antiviral therapies have not yielded a successful solution hitherto. The CRISPR-Cas9 system represents a promising tool for eliminating viral infections. In this review, we highlight 1) the recent progress of CRISPR-Cas technology in decoding and diagnosis of viral outbreaks, 2) its applications to eliminate viral infections in both pre-integration and provirus stages, and 3) various delivery systems that are employed to introduce the platform into target cells.
成簇规律间隔短回文重复序列（CRISPR）相关蛋白核酸酶（Cas）被鉴定为古细菌和细菌中的一种适应性免疫系统。该系统的II型，即CRISPR-Cas9，是最通用的形式，能够实现简便高效的靶向基因组编辑。病毒感染对全球健康有严重影响，而传统的抗病毒疗法迄今尚未取得成功的解决方案。CRISPR-Cas9系统是消除病毒感染的一种有前景的工具。在本综述中，我们重点介绍了：1）CRISPR-Cas技术在病毒爆发解码和诊断方面的最新进展；2）其在病毒前整合和前病毒阶段消除病毒感染的应用；3）用于将该平台导入靶细胞的各种递送系统。
