Biotechnology Process Engineering Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB), 30 Yeongudanji-ro, Cheongwon-gu, Cheongju-si, Chungcheongbuk-do, 28116, Republic of Korea.
Department of Bioprocess Engineering, KRIBB School of Biotechnology, Korea University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon, 34113, Republic of Korea.
Appl Microbiol Biotechnol. 2019 Feb;103(4):1545-1555. doi: 10.1007/s00253-018-9571-7. Epub 2019 Jan 3.
Sustainable manufacture of dicarboxylic acids (DCAs), which are used as raw materials for multiple commercial products, has been an area of considerable research interest in recent years. Traditional chemical-based manufacture of DCAs suffers from limitations such as harsh operational conditions and generation of hazardous by-products. Microbiological methods involving DCA production depend on the capability of alkane-assimilating microorganisms, particularly α, ω-oxidation, to metabolize alkanes. Alkanes are still used as the most common substrates for this method, but the use of renewable resources, such as vegetable oil-derived fatty acid methyl esters (FAMEs), offers multiple advantages for the sustainable production of DCA. However, DCA production using FAME, unlike that using alkanes, still has low productivity and process stability, and we have attempted to identify several limiting factors that weaken the competitiveness. This review discusses the current status and suggests solutions to various obstacles to improve the biotransformation process of FAMEs.
近年来，作为多种商业产品原料的二酸（DCAs）的可持续制造一直是相当多研究关注的领域。传统的基于化学的 DCA 制造受到苛刻的操作条件和危险副产物生成等限制。涉及 DCA 生产的微生物方法取决于烷烃同化微生物的能力，特别是 α、ω-氧化，以代谢烷烃。烷烃仍然是该方法最常用的底物，但使用可再生资源，如植物油衍生的脂肪酸甲酯（FAMEs），为 DCA 的可持续生产提供了多种优势。然而，与使用烷烃不同，使用 FAME 生产 DCA 的方法仍然存在生产力和过程稳定性低的问题，我们试图确定削弱竞争力的几个限制因素。本综述讨论了当前的状况，并提出了解决各种障碍的建议，以改善 FAME 的生物转化过程。
