Department of Food Science and Engineering, Ewha Womans University, Seoul 03760, Republic of Korea.
Department of Biochemical Engineering, Gangneung-Wonju National University, Gangneung 25457, Republic of Korea.
Bioresour Technol. 2018 Mar;251:288-294. doi: 10.1016/j.biortech.2017.12.036. Epub 2017 Dec 15.
Oils and fatty acids are important renewable resources provided by nature. Therefore, biotransformation of renewable oils and fatty acids into industrially relevant C9 chemicals was investigated in this study. Olive oil, soybean oil, yeast derived oil, and microalgae fatty acid methyl esters were converted into n-nonanoic acid, 9-hydroxynonanoic acid, and 1,9-nonanedioic acid by a lipase and a recombinant Escherichia coli expressing oleate hydratase, long chain secondary alcohol dehydrogenase, Baeyer-Villiger monooxygenase, long chain primary alcohol dehydrogenase, and aldehyde dehydrogenase. It was found that n-nonanoic acid and azelaic acid could be produced to a concentration of 4.3 mM from 3 g/L olive oil with a specific product formation rate of 3.1 U/g dry cells. Biotransformation rates were influenced by compositions of fatty acids and purity of the starting material. This study may contribute to the production of industrially relevant C9 chemicals from renewable oils and fatty acids by simultaneous enzyme/whole-cell biotransformation.
油脂和脂肪酸是自然界提供的重要可再生资源。因此，本研究考察了将可再生油脂和脂肪酸生物转化为工业相关的 C9 化学品。通过脂肪酶和表达油酸水解酶、长链仲醇脱氢酶、Baeyer-Villiger 单加氧酶、长链伯醇脱氢酶和醛脱氢酶的重组大肠杆菌，将橄榄油、大豆油、酵母来源的油和微藻脂肪酸甲酯转化为壬酸、9-羟基壬酸和 1,9-壬二酸。结果发现，从 3g/L 的橄榄油中可以产生浓度为 4.3mM 的壬酸和壬二酸，比活为 3.1U/g 干细胞。生物转化速率受脂肪酸组成和起始原料纯度的影响。本研究可能有助于通过同时的酶/全细胞生物转化，从可再生油脂和脂肪酸生产工业相关的 C9 化学品。
