菊科植物菊花（Chrysanthemum morifolium L.）和向日葵（Helianthus annuus L.）中同源异型AP3进化枝MADS盒基因的多样化
Diversification of the Homeotic AP3 Clade MADS-Box Genes in Asteraceae Species Chrysanthemum morifolium L. and Helianthus annuus L.
作者信息
Shchennikova A V, Shulga O A, Skryabin K G
机构信息
Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, 119071, Russia.
All-Russia Research Institute of Agricultural Biotechnology, Moscow, 127550, Russia.
出版信息
Dokl Biochem Biophys. 2018 Nov;483(1):348-354. doi: 10.1134/S1607672918060145. Epub 2019 Jan 3.
The structure and phylogeny of MADS-box genes HAM91 of sunflower (Helianthus annuus) and CDM115 of chrysanthemum (Chrysanthemum morifolium) were characterized. It is shown that these genes encode MADS-domain transcription factors, which are orthologs of TM6 (Solanum lycopersicum) and APETALA3 (Arabidopsis thaliana), respectively. We obtained two types of transgenic tobacco plants (Nicotiana tabacum) with constitutive expression of HAM91 and CDM115 genes. Both types of plants flowered later than the control plants and formed more flowers and seed pods. The weight of seeds of 35S::CDM115 plants was significantly lower than in the control and 35S::HAM91 plants, which may indicate to a change in the identity of ovules in 35S::CDM115.
对向日葵（Helianthus annuus）的MADS-box基因HAM91和菊花（Chrysanthemum morifolium）的CDM115的结构和系统发育进行了表征。结果表明，这些基因编码MADS结构域转录因子，它们分别是番茄（Solanum lycopersicum）的TM6和拟南芥（Arabidopsis thaliana）的APETALA3的直系同源物。我们获得了两种组成型表达HAM91和CDM115基因的转基因烟草植株（Nicotiana tabacum）。这两种类型的植株开花时间均晚于对照植株，且形成了更多的花和种荚。35S::CDM115植株的种子重量显著低于对照和35S::HAM91植株，这可能表明35S::CDM115中胚珠的特性发生了变化。
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