Shchennikova A V, Shulga O A, Skryabin K G
Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, 119071, Russia.
All-Russia Research Institute of Agricultural Biotechnology, Moscow, Russia.
Dokl Biochem Biophys. 2018 Nov;483(1):363-368. doi: 10.1134/S1607672918060182. Epub 2019 Jan 3.
The structure of the MADS-box gene HAM31 of the sunflower (Helianthus annuus) was characterized. It is shown that the product of this gene is an ortholog of the B-class MADS transcription factor PISTILLATA (Arabidopsis thaliana). Two types of transgenic tobacco plants (Nicotiana tabacum) with the constitutive expression of the HAM31 gene in the sense and antisense orientation were obtained. The 35S::HAM31s plants formed flowers with an altered gynoecium identity, whereas 35S::HAM31as plants did not differ from the control.
对向日葵（Helianthus annuus）的MADS盒基因HAM31的结构进行了表征。结果表明，该基因的产物是B类MADS转录因子PISTILLATA（拟南芥）的直系同源物。获得了两种在正义和反义方向上组成型表达HAM31基因的转基因烟草植株（Nicotiana tabacum）。35S::HAM31s植株形成了具有改变的雌蕊特征的花，而35S::HAM31as植株与对照没有差异。
