Mi Ze-Yuan, Zhao Qian, Lu Chan, Zhang Qian, Li Lin, Liu Shuai, Wang Shi-Qiang, Wang Zhe-Zhi, Niu Jun-Feng
National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, Xi'an 710119, China.
Plants (Basel). 2021 Oct 14;10(10):2184. doi: 10.3390/plants10102184.
(Thunb. ex A. Murray) Rchb. f., a species of the perennial herb Orchidaceae, has potent anti-inflammatory and antiviral biological activities. MADS-box transcription factors play critical roles in the various developmental processes of plants. Although this gene family has been extensively investigated in many species, it has not been analyzed for . In total, 45 MADS-box genes were identified from in this study, which were classified into five subfamilies (Mδ, MIKC, Mα, Mβ, and Mγ). Meanwhile, the highly correlated protein domains, motif compositions, and exon-intron structures of were investigated according to local databases. Chromosome distribution and synteny analyses revealed that segmental duplication and homologous exchange were the main expansion mechanisms. Further, RT-qPCR analysis revealed that had different expression patterns in response to various stress treatments. Our results provide a potential theoretical basis for further investigation of the functions of MADS genes during the growth of .
(拇指。前A.默里)Rchb。f.,一种多年生草本兰科植物,具有强大的抗炎和抗病毒生物活性。MADS-box转录因子在植物的各种发育过程中起关键作用。尽管该基因家族已在许多物种中得到广泛研究,但尚未对其进行分析。在本研究中,总共从[具体物种]中鉴定出45个MADS-box基因,它们被分为五个亚家族(Mδ、MIKC、Mα、Mβ和Mγ)。同时,根据本地[数据库名称]数据库研究了[具体物种]的高度相关蛋白质结构域、基序组成和外显子-内含子结构。染色体分布和共线性分析表明,片段重复和同源交换是[具体物种]基因扩展的主要机制。此外,RT-qPCR分析表明,[具体物种]在不同胁迫处理下具有不同的表达模式。我们的结果为进一步研究MADS基因在[具体物种]生长过程中的功能提供了潜在的理论基础。