Department of Dermatology and Plastic Surgery, Akita University Graduate School of Medicine, Akita, Japan.
Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
PLoS One. 2019 Jan 4;14(1):e0210013. doi: 10.1371/journal.pone.0210013. eCollection 2019.
Epidermal ceramides are indispensable lipids that maintain the functions of the stratum corneum. Esterified omega-hydroxyacyl-sphingosine (EOS) ceramide with a linoleate moiety is one of the most important ceramide species for forming cornified lipid envelopes. This linoleate moiety is eventually metabolized to trihydroxy-linoleic acid (triol, 9,10,13-trihydroxy-11E-octadecenoic acid). Thus, we assumed that a decrease of triols might reflect skin barrier dysfunction. Against this background, the purposes of this study were to measure the triols by a simple tape-stripping method and to determine the correlation between the amount of triols and transepidermal water loss (TEWL) as an indicator of barrier dysfunction in atopic dermatitis patients. Twenty Japanese subjects with normal skin and 20 atopic dermatitis patients were enrolled in this study. TEWL was measured and triols of the stratum corneum were analyzed by tape-stripping. The results showed for the first time that triols in the stratum corneum could be simply measured using the tape-stripping method. The triol levels in atopic dermatitis patients were much higher than those in healthy subjects. Moreover, the triol levels correlated with TEWL of non-lesional forearm skin in patients with atopic dermatitis. The results suggest that the assaying of triol levels via non-invasive tape-stripping could be beneficial for monitoring barrier function in atopic dermatitis.
表皮神经酰胺是维持角质层功能所必需的脂质。具有亚油酸部分的酯化 ω-羟基酰基神经酰胺是形成角蛋白脂质包膜的最重要的神经酰胺种类之一。这个亚油酸部分最终被代谢为三羟基亚麻酸（三醇，9,10,13-三羟基-11E-十八碳烯酸）。因此，我们假设三醇的减少可能反映了皮肤屏障功能障碍。在此背景下，本研究的目的是通过简单的胶带剥离法测量三醇，并确定三醇的量与经皮水分流失（TEWL）之间的相关性，TEWL 是特应性皮炎患者屏障功能障碍的指标。本研究纳入了 20 名日本正常皮肤受试者和 20 名特应性皮炎患者。测量 TEWL 并通过胶带剥离分析角质层中的三醇。结果首次表明，可通过胶带剥离法简单测量角质层中的三醇。特应性皮炎患者的三醇水平明显高于健康受试者。此外，特应性皮炎患者非病变前臂皮肤的三醇水平与 TEWL 相关。这些结果表明，通过非侵入性胶带剥离法测定三醇水平可能有助于监测特应性皮炎的屏障功能。
