Wang Dapeng, Chen Ying, Song Xue, Wu Shuang, Zhang Na, Zheng Fei, Yang Guangrun
Department of Radiotherapy, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar, China.
Transl Cancer Res. 2022 May;11(5):1255-1268. doi: 10.21037/tcr-22-855.
Long non-coding RNAs (lncRNAs) are involved in various biological processes in non-small cell lung cancer (NSCLC). This study aimed to investigate the key lncRNA axis in the development of NSCLC and its potential molecular mechanism.
LncRNA is considered to be a competitive endogenous RNA (ceRNA) and its potential targeting microRNAs (miRNAs) were predicted through LncBase predicted v.2. The expression of and in NSCLC tissues and cells was detected by reverse transcription polymerase chain reaction (RT-PCR). The Cell Counting Kit-8 (CCK-8) and cell colony-forming test were used to detect the effect of cell proliferation. RT-PCR was used to detect the expression changes of and . Western Blot was used to detect the changes of the cell cycle protein. Dual luciferase activity was used to determine the potential mechanism of lncRNA .
LncRNA was highly expressed and was lowly expressed in NSCLC tissues and cell lines. LncBase predicted v.2.0 reported a high-scoring binding between and . The luciferase reporter assay defined the regulatory relationship between and . In NSCLC cells, knockdown of significantly increased the expression of , decreased the proliferation and colony formation number of cancer cells, and reduced the expression of and . Meanwhile, overexpression of significantly inhibited the cell proliferation and colony formation number, and reduced the expression of and . Furthermore, according to the results of the dual-luciferase activity assay, targeted the 3' untranslated regions (3' UTRs) of , validating that is a direct target of . Overexpression of restored the role of in NSCLC cells.
LncRNA can regulate the proliferation of NSCLC cells via / signaling. Therefore, may act as a prospective biomarker and therapeutic target for patients with NSCLC.
长链非编码RNA（lncRNAs）参与非小细胞肺癌（NSCLC）的多种生物学过程。本研究旨在探讨NSCLC发生发展过程中的关键lncRNA轴及其潜在分子机制。
lncRNA被认为是一种竞争性内源性RNA（ceRNA），通过LncBase预测v.2预测其潜在靶向的微小RNA（miRNAs）。采用逆转录聚合酶链反应（RT-PCR）检测NSCLC组织和细胞中lncRNA及miRNA的表达。使用细胞计数试剂盒-8（CCK-8）和细胞集落形成试验检测细胞增殖情况。RT-PCR检测相关基因表达变化。蛋白质免疫印迹法检测细胞周期蛋白变化。双荧光素酶活性检测确定lncRNA的潜在作用机制。
lncRNA在NSCLC组织和细胞系中高表达，而miRNA低表达。LncBase预测v.2.0报告lncRNA与miRNA之间存在高评分结合。荧光素酶报告基因检测确定了两者之间的调控关系。在NSCLC细胞中，敲低lncRNA显著增加miRNA表达，降低癌细胞增殖和集落形成数量，并降低相关基因表达。同时，过表达miRNA显著抑制细胞增殖和集落形成数量，并降低相关基因表达。此外，根据双荧光素酶活性检测结果，miRNA靶向lncRNA的3'非翻译区（3' UTRs），证实lncRNA是miRNA的直接靶点。过表达lncRNA恢复了miRNA在NSCLC细胞中的作用。
lncRNA可通过miRNA/信号通路调节NSCLC细胞增殖。因此，lncRNA可能作为NSCLC患者的潜在生物标志物和治疗靶点。
