微小 RNA-886 抑制骨肉瘤细胞增殖，其成熟受长链非编码 RNA OXCT1-AS1 抑制。

MicroRNA-886 suppresses osteosarcoma cell proliferation and its maturation is suppressed by long non-coding RNA OXCT1-AS1.

机构信息

Joint Surgery Department, First People's Hospital of Shangqiu City, Shangqiu City, Henan Province, China.

General Medicine Department, First People's Hospital of Shangqiu City, Shangqiu City, Henan Province, China.

出版信息

Bioengineered. 2022 Mar;13(3):5769-5778. doi: 10.1080/21655979.2022.2031669.

This study aimed to investigate the roles of microRNA-886 (miR-886) and long non-coding RNA (lncRNA) OXCT1-AS1 in osteosarcoma (OS). We predicted that they might interact with each other. The expression of OXCT1-AS1 and miR-886 (mature and premature) in osteosarcoma and paired non-tumor tissues from 66 OS patients was negatively correlated. Overexpression and silencing assays showed that OXCT1-AS1 suppresses miR-886 maturation. RNA-RNA pulldown and subcellular fractionation assays demonstrated the direct interaction between OXCT1-AS1 and miR-886. BrdU proliferation assays revealed that OXCT1-AS1 promoted OS cell proliferation, and miR-886 reduced the enhancing effects of OXCT1-AS1 on OS cell proliferation. Western blot showed that OXCT1-AS1 had no effects on the levels of epithelial-mesenchymal transition biomarkers. Overall, OXCT1-AS1 suppresses miR-886 maturation to promote OS cell proliferation.

本研究旨在探讨微小 RNA-886（miR-886）和长链非编码 RNA（lncRNA）OXCT1-AS1 在骨肉瘤（OS）中的作用。我们预测它们可能相互作用。对 66 例骨肉瘤患者的骨肉瘤和配对非肿瘤组织中的 OXCT1-AS1 和 miR-886（成熟和前体）的表达进行了检测，结果呈负相关。过表达和沉默实验表明，OXCT1-AS1 抑制 miR-886 的成熟。RNA-RNA 下拉和亚细胞分馏实验证明了 OXCT1-AS1 与 miR-886 之间的直接相互作用。BrdU 增殖实验表明，OXCT1-AS1 促进 OS 细胞增殖，而 miR-886 降低了 OXCT1-AS1 对 OS 细胞增殖的增强作用。Western blot 表明，OXCT1-AS1 对上皮-间充质转化标志物的水平没有影响。总的来说，OXCT1-AS1 抑制 miR-886 的成熟，从而促进 OS 细胞增殖。