Ham Baknoon, Kim Myungsuk, Son Yang-Ju, Chang Sungyul, Jung Sang Hoon, Nho Chu Won, Kwon Man Jae
Graduate School of Energy and Environment, Korea University, Seoul, South Korea.
Natural Products Research Center, Korea Institute of Science and Technology, Gangneung, South Korea.
Planta Med. 2019 May;85(7):599-607. doi: 10.1055/a-0824-1282. Epub 2019 Jan 4.
has been traditionally used as a medicinal plant owing to its anti-inflammatory, anthelmintic, tonic, antihyperglycemic, and diaphoretic properties. We hypothesized that might have therapeutic potential in alleviating allergy and atopic dermatitis. Thus, we investigated the inhibitory effects of extract against allergic responses and 2,4-dinitrochlorobenzene-induced atopic dermatitis-like dorsal skin lesions using immunoglobulin E-sensitized rat basophilic leukemia-2H3 mast cells and NC/Nga mice. Degranulation and enzyme-linked immunosorbent assays were conducted to measure degranulation, proinflammatory cytokine levels, and prostaglandin E concentrations in immunoglobulin E/antigen-sensitized RBL-2H3 mast cells. The therapeutic efficacy of extract in 2,4-dinitrochlorobenzene-induced atopic dermatitis was evaluated through morphological, physiological, and immunological analysis. extract inhibited -hexosaminidase and histamine release and reduced tumor necrosis factor-, interleukin-4, and prostaglandin E secretion. Furthermore, extract suppressed atopic dermatitis-like skin lesions by regulating the serum levels of immunoglobulin E and immunoglobulin G2a, and messenger ribonucleic acid expression of T helper cell 1- and T helper cell 2-related mediators in the skin lesions. Histopathological analyses showed a decrease in epidermal thickness and intradermal inflammatory cell infiltration. These results suggested that extract might have beneficial effects in treating allergic and atopic dermatitis-like skin disorders.
由于其具有抗炎、驱虫、滋补、抗高血糖和发汗特性，传统上一直被用作药用植物。我们推测[具体植物名称]可能在减轻过敏和特应性皮炎方面具有治疗潜力。因此，我们使用免疫球蛋白E致敏的大鼠嗜碱性白血病-2H3肥大细胞和NC/Nga小鼠，研究了[具体植物名称]提取物对过敏反应和2,4-二硝基氯苯诱导的特应性皮炎样背部皮肤损伤的抑制作用。进行了脱颗粒和酶联免疫吸附测定，以测量免疫球蛋白E/抗原致敏的RBL-2H3肥大肥大细胞中的脱颗粒、促炎细胞因子水平和前列腺素E浓度。通过形态学、生理学和免疫学分析评估了[具体植物名称]提取物对2,4-二硝基氯苯诱导的特应性皮炎的治疗效果。[具体植物名称]提取物抑制了β-己糖胺酶和组胺释放，并降低了肿瘤坏死因子-α、白细胞介素-4和前列腺素E的分泌。此外，[具体植物名称]提取物通过调节皮肤损伤中免疫球蛋白E和免疫球蛋白G2a的血清水平以及辅助性T细胞1和辅助性T细胞2相关介质的信使核糖核酸表达，抑制了特应性皮炎样皮肤损伤。组织病理学分析显示表皮厚度和真皮内炎性细胞浸润减少。这些结果表明，[具体植物名称]提取物可能对治疗过敏性和特应性皮炎样皮肤疾病具有有益作用。
