Haemal Internal Medicine, Linyi Central Hospital, Yishui County, Linyi, Shandong 276400, PR China.
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE; Shandong Key Laboratory of Biochemical Analysis; Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong; College of Chemistry and Molecular Engineering. Qingdao University of Science and Technology, Qingdao 266042, PR China.
Talanta. 2019 Mar 1;194:658-663. doi: 10.1016/j.talanta.2018.10.098. Epub 2018 Oct 30.
In this study, a novel strategy for amplified electrochemiluminescence (ECL) detection of DNA was proposed based on excellent ECL activity of graphene quantum dots (GQDs) coupled with multiple cycling amplification technique. A new type of graphene QDs with well ECL property and uniform size were firstly synthesized, then the graphene QDs were assembled on the electrode by poly (diallyldimethylammonium chloride) (PDDA)-graphene oxide (GO) nanocomposites, which could greatly improve ECL signal and stability of QDs. A novel signal-on ECL biosensor for DNA analysis was designed by using ECL quenching of gold nanoparticles (NPs) to graphene QDs combined with endonuclease-aided cyclic amplification strategy. As a result, the proposed strategy can be conveniently expanded to other biosensing application, especially in clinical diagnoses.
在本研究中，提出了一种基于石墨烯量子点（GQDs）的优异电化学发光（ECL）活性与多重循环扩增技术相结合的新型 DNA 放大电化学发光（ECL）检测策略。首先合成了具有良好 ECL 性能和均匀尺寸的新型石墨烯量子点，然后通过聚二烯丙基二甲基氯化铵（PDDA）-氧化石墨烯（GO）纳米复合材料将石墨烯量子点组装在电极上，这可以大大提高量子点的 ECL 信号和稳定性。通过将金纳米粒子（NPs）对石墨烯量子点的 ECL 猝灭与内切酶辅助循环扩增策略相结合，设计了一种新型的用于 DNA 分析的信号开启型 ECL 生物传感器。因此，该策略可以方便地扩展到其他生物传感应用，特别是在临床诊断中。
