School of Pharmaceutical Sciences and Chongqing Key Laboratory of Natural Drug Research, Chongqing University, 55 South Daxuecheng Road, Chongqing 401331, PR China.
First Affiliated Hospital of the Medical College, Shihezi University, Xinjiang 832008, PR China.
Talanta. 2019 Mar 1;194:79-85. doi: 10.1016/j.talanta.2018.09.109. Epub 2018 Oct 9.
Superoxide anion is widely involved in broad cellular functions such as immune response, cell signaling and cell proliferation in regulated concentrations but are implicated in the development of various disease in uncontrolled levels. It is highly desired to develop an efficient analytical strategy to determine the concentration of O with high selectivity and sensitivity. In our work, a novel fluorescence probe was developed to selectively detect superoxide anions. This probe demonstrated the following advantages: (1) a low detection limit and wide linear region from nanomolar to micromolar concentrations; (2) two-channel responsiveness; and (3) targeting mitochondria specifically. Moreover, the probe was successfully applied to visualize superoxide anions in vitro and in vivo. These excellent features could enable its sensitive determination for O in biological systems and promising roles in biomedical applications.
超氧阴离子广泛参与多种细胞功能，如免疫反应、细胞信号转导和细胞增殖，在调节浓度下发挥作用，但在不受控制的水平下与多种疾病的发展有关。因此，人们非常希望开发一种有效的分析策略，以高选择性和灵敏度来确定 O 的浓度。在我们的工作中，开发了一种新型荧光探针来选择性检测超氧阴离子。该探针具有以下优点：(1)检测限低，线性范围从纳摩尔到微摩尔浓度；(2)双通道响应；(3)特异性靶向线粒体。此外，该探针成功地用于体外和体内超氧阴离子的可视化。这些优异的特性使其能够在生物系统中对 O 进行灵敏测定，并在生物医学应用中具有广阔的应用前景。
