Li Jiahao, Wang Xin, Shan Yanke, Huang Huachuan, Jian Dan, Xue Liang, Wang Shouyu, Liu Fei
Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education & Single Molecule Nanometry Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
School of Manufacturing Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, Sichuan, China.
Micromachines (Basel). 2019 Jan 1;10(1):27. doi: 10.3390/mi10010027.
Tetracycline is widely used as medicine for disease treatments and additives in animal feeding. Unfortunately, the abuse of tetracycline inevitably causes tetracycline residue in animal-origin foods. Though classical methods can detect tetracycline in high sensitivity and precision, they often rely on huge and expensive setups as well as complicated and time-consuming operations, limiting their applications in rapid and on-site detection. Here, we propose a handheld inkjet printing paper chip based smart tetracycline detector: tetracycline can be determined by inkjet printing prepared paper chip based enzyme-linked immunosorbent assay (ELISA) with the advantages of high sensitivity, excellent specificity and low cost; moreover, a smartphone based paper chip reader and application is designed for automatically determining tetracycline with simple operations, high precision and fast speed. The smart tetracycline detector with a compact size of 154 mm × 80 mm × 50 mm and self-supplied internal power can reach a rather low detection limit of ~0.05 ng/mL, as proved by practical measurements. It is believed the proposed handheld inkjet printing paper chip based smart tetracycline detector is a potential tool in antibiotic sensing for routine uses at home and on-site detection in the field.
四环素被广泛用作疾病治疗药物和动物饲料添加剂。不幸的是，四环素的滥用不可避免地导致动物源食品中出现四环素残留。尽管传统方法能够高灵敏度、高精度地检测四环素，但它们通常依赖庞大且昂贵的设备以及复杂耗时的操作，这限制了它们在快速现场检测中的应用。在此，我们提出一种基于手持喷墨打印纸芯片的智能四环素检测仪：通过喷墨打印制备的纸芯片酶联免疫吸附测定法（ELISA）可测定四环素，具有高灵敏度、优异特异性和低成本的优点；此外，还设计了一种基于智能手机的纸芯片阅读器及应用程序，用于以简单操作、高精度和快速速度自动测定四环素。经实际测量证明，尺寸紧凑为154毫米×80毫米×50毫米且自带内部电源的智能四环素检测仪能够达到相当低的检测限，约为0.05纳克/毫升。相信所提出的基于手持喷墨打印纸芯片的智能四环素检测仪是一种在家中日常使用和现场检测抗生素传感方面的潜在工具。
