Chen Yonghe, Su Baochang, Shang Mengqiao
Xintang Hospital of Zengcheng District, Guangzhou 511340, China.
Affiliated Hospital of Jinan University, Guangzhou 510000, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2018 Dec 30;38(12):1453-1458. doi: 10.12122/j.issn.1673-4254.2018.12.09.
To study the diagnostic value of P2X7 receptor for rheumatoid arthritis (RA) and its role in the inflammatory response.
With the synovial tissues from 25 patients with bone and joint replacement as the control,the synovial tissues of 25 RA patients were examined for the relative expression of P2X7 receptor mRNA using qRT-PCR.In an immortalized RA synovial cell line (MH7A),the effect of P2X7 receptor knockdown via a small interfering RNA were examined on the productions of the inflammatory cytokines including interleukin-1β(IL-1β),IL-6,and IL-8 using ELISA.
The RA patients showed significantly higher levels of P2X7 receptor mRNA expression in the synovial tissue than the control patients.P2X7 receptor had a good diagnostic value for RA.The expression levels of IL-1β,IL-6,and IL-8 were positively correlated with the levels of P2X7 receptor in the synovial tissues of RA patients (<0.001).In MH7A cells,P2X7 receptor knockdown obviously reduced the secretion of IL-1β and IL-6.
RA patients show elevated P2X7 receptor level in the synovial tissue, which has a good diagnostic value for RA.Blocking P2X7 receptor can inhibit inflammatory factor secretion and suppress inflammatory reactions.
研究P2X7受体对类风湿关节炎（RA）的诊断价值及其在炎症反应中的作用。
以25例进行骨关节置换患者的滑膜组织作为对照，采用qRT-PCR检测25例RA患者滑膜组织中P2X7受体mRNA的相对表达水平。在永生化的RA滑膜细胞系（MH7A）中，通过小干扰RNA敲低P2X7受体，采用ELISA检测其对白细胞介素-1β（IL-1β）、IL-6和IL-8等炎性细胞因子产生的影响。
RA患者滑膜组织中P2X7受体mRNA表达水平显著高于对照患者。P2X7受体对RA具有良好的诊断价值。RA患者滑膜组织中IL-1β、IL-6和IL-8的表达水平与P2X7受体水平呈正相关（<0.001）。在MH7A细胞中，敲低P2X7受体明显降低了IL-1β和IL-6的分泌。
RA患者滑膜组织中P2X7受体水平升高，对RA具有良好的诊断价值。阻断P2X7受体可抑制炎性因子分泌，抑制炎症反应。
