lncRNA PTENP1的过表达在体外抑制胶质瘤细胞的增殖和转移。

Overexpression of lncRNA PTENP1 suppresses glioma cell proliferation and metastasis in vitro.

作者信息

Hu Su, Xu Li, Li Lihua, Luo Dongdong, Zhao Hailin, Li Dan, Peng Biao

机构信息

Department of Neurosurgery, The Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou 510095, Guangdong, People's Republic of China,

Department of Neurosurgery, The Central People's Hospital of Zhanjiang, Zhanjiang 524045, Guangdong, People's Republic of China.

出版信息

Onco Targets Ther. 2018 Dec 21;12:147-156. doi: 10.2147/OTT.S182537. eCollection 2019.

BACKGROUND

Glioma is one of the most common malignancies of the central nervous system in adults. The lncRNA PTEN pseudogene-1 (PTENP1) has been reported to play an important role in the development and progression of various cancers. However, the molecular mechanism by which lncRNA PTENP1 affects the development and progression of gliomas remains unclear.

MATERIALS AND METHODS

The levels of PTENP1 expression in glioma tissues and normal brain tissues were detected by quantitative real-time PCR. Cell Counting Kit-8 and 5-ethynyl-2'-deoxyuridine staining assays were performed to detect cell proliferation. Flow cytometry was used to analyze cell cycle progression. Transwell assay and scratch test were used to detect cell migration and invasion, and Western blot studies were performed to detect protein expression.

RESULTS

Our results showed that expression of lncRNA PTENP1 was decreased in glioma tissues when compared with normal brain tissues. Overexpression of PTENP1 suppressed SHG44 and U251 cell proliferation and significantly decreased the numbers of S-phase cells. Furthermore, the invasion and migration abilities of SHG44 and U251 cells were reduced after being transfected with a PTENP1 overexpression plasmid. Overexpression of PTENP1 induced the expression of p21 protein and suppressed the p38 signaling pathway.

CONCLUSION

Our study investigated the function of PTENP1 in glioma and provided new insights for treating that malignancy.

背景

胶质瘤是成人中枢神经系统最常见的恶性肿瘤之一。据报道，长链非编码RNA PTEN假基因-1（PTENP1）在多种癌症的发生和发展中起重要作用。然而，lncRNA PTENP1影响胶质瘤发生和发展的分子机制仍不清楚。

材料与方法

采用定量实时PCR检测胶质瘤组织和正常脑组织中PTENP1的表达水平。进行细胞计数试剂盒-8和5-乙炔基-2'-脱氧尿苷染色试验以检测细胞增殖。流式细胞术用于分析细胞周期进程。Transwell试验和划痕试验用于检测细胞迁移和侵袭，并进行蛋白质印迹研究以检测蛋白质表达。

结果

我们的结果表明，与正常脑组织相比，lncRNA PTENP1在胶质瘤组织中的表达降低。PTENP1的过表达抑制了SHG44和U251细胞的增殖，并显著减少了S期细胞的数量。此外，用PTENP1过表达质粒转染后，SHG44和U251细胞的侵袭和迁移能力降低。PTENP1的过表达诱导了p21蛋白的表达并抑制了p38信号通路。