Qian Yu-Yuan, Li Kun, Liu Quan-Yan, Liu Zhi-Su
Department of Hepatobiliary and Pancreatic Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.
Oncotarget. 2017 Nov 6;8(64):107859-107869. doi: 10.18632/oncotarget.22305. eCollection 2017 Dec 8.
Long non-coding RNA PTENP1, the pseudogene of PTEN tumor suppressor, was previously reported to be a tumour suppressor in some cancer types. However, the precise effects mediated by PTENP1 transcripts within intricate regulatory networks involving molecular interactions with PTEN and tumorigenicity in hepatocellular carcinoma (HCC) remains elusive. Here, we identify the critical biological functions of PTENP1 and discuss whether PTENP1 could directly interact with miR-193a-3p to affect the progression of HCC both and . We demonstrated that PTENP1 level in the HCC tissues was significantly lower compared with those in the adjacent normal tissues. And PTENP1 was able to repress cell invasion, metastasis, and proliferation capacity in HCC cell lines. The overexpression of PTENP1 inhibited HCC growth both and . There were a binding sequence and direct interaction between PTENP1 and miR-193a-3p. PTENP1 as an endogenous sponge interacted with miR-193a-3p, leading to regulate the downstream PTEN/Akt pathway. These results suggested that PTENP1 with its suppression effect might serve as novel biomarkers and potent therapeutic strategies in HCC.
长链非编码RNA PTENP1是抑癌基因PTEN的假基因，此前有报道称其在某些癌症类型中是一种肿瘤抑制因子。然而，在涉及与PTEN分子相互作用和肝细胞癌（HCC）致瘤性的复杂调控网络中，PTENP1转录本介导的精确作用仍不清楚。在此，我们确定了PTENP1的关键生物学功能，并讨论了PTENP1是否能直接与miR-193a-3p相互作用，从而在体内和体外影响HCC的进展。我们证明，HCC组织中PTENP1水平与相邻正常组织相比显著降低。并且PTENP1能够抑制HCC细胞系中的细胞侵袭、转移和增殖能力。PTENP1的过表达在体内和体外均抑制HCC生长。PTENP1与miR-193a-3p之间存在一个结合序列和直接相互作用。PTENP1作为一种内源性海绵与miR-193a-3p相互作用，从而调节下游PTEN/Akt通路。这些结果表明，具有抑制作用的PTENP1可能作为HCC的新型生物标志物和有效的治疗策略。
