Department of Plant sciences, Quaid-i-Azam University, Islamabad, Islamabad Capital Territory, Pakistan.
Department of Animal Sciences, Quaid-i-Azam University, Islamabad, Islamabad Capital Territory, Pakistan.
Microsc Res Tech. 2019 Sep;82(9):1401-1409. doi: 10.1002/jemt.23292. Epub 2019 May 20.
The research was performed to investigate pollen morphology of endangered species of Capparidaceae in subtropical regions of Pakistan. The distinguishing characters were investigated by using light microscope and scanning electron microscope. Palynological study is comprised of pollen shape, pollen type, exine sculpturing, polar and equatorial diameter, length and width of colpi, mesocolpium, and exine thickness. In polar view, Cleome viscosa exhibited the highest pollen size 26.4 (32.7-24.5 μm) ±0.776 whereas Capparis spinosa appeared to be the lowest 12.6 (14.5-10.7 μm) ±0.400. In equatorial view, Cleome viscosa had the largest pollen size 17.1 (20.0-15.0 μm) ±0.606 and Capparis spinosa had the smallest pollen size 9.7 (12.50-8.00 μm) ±0.394. The maximum fertility percentage has been observed in Capparis spinosa, that is, 98.96% and minimum in Cleome viscosa, that is, 82.93%. Diagnostic key has been constructed to state the essential diagnostic features by means of which the taxa can be identified. Remarkable variations have been observed in pollen size, shape, and exine sculpturing. All the selected species were tricolporate. Prolate to subprolate pollen were observed. There is a great variation existed in exine sculpturing such as in Capparis decidua and C. sp. nova sculpturing is reticulate, in Capparis himalayensis sculpturing is Scabrate granulate, in Capparis spinosa sculpturing is Psilate, in Cleome viscosa sculpturing is regulate-reticulate, in Dipterygium glaucum sculpturing is regulate and in Gynandropsis gynandra sculpturing is striate-regulate. On the basis of overall characteristics of pollen it seems that palynology of this family is helpful at the generic and specific level.
这项研究旨在调查巴基斯坦亚热带地区濒危卡帕拉科植物的花粉形态。使用光学显微镜和扫描电子显微镜研究了鉴别特征。花粉学研究包括花粉形状、花粉类型、外壁纹饰、极轴和赤道直径、裂缝的长度和宽度、中槽和外壁厚度。在极视图中，Cleome viscosa 的花粉大小最高，为 26.4（32.7-24.5μm）±0.776，而 Capparis spinosa 的花粉大小最低，为 12.6（14.5-10.7μm）±0.400。在赤道视图中，Cleome viscosa 的花粉最大，为 17.1（20.0-15.0μm）±0.606，而 Capparis spinosa 的花粉最小，为 9.7（12.50-8.00μm）±0.394。Capparis spinosa 的最大可育百分比为 98.96%，Cleome viscosa 的最小可育百分比为 82.93%。已经构建了一个诊断关键，通过该关键可以通过其特征来识别分类群。观察到花粉大小、形状和外壁纹饰的显著变化。所有选定的物种都是三孔的。观察到长形到亚长形的花粉。外壁纹饰存在很大的差异，例如 Capparis decidua 和 C. sp. nova 的纹饰是网状的，Capparis himalayensis 的纹饰是粒状粗糙的，Capparis spinosa 的纹饰是光滑的，Cleome viscosa 的纹饰是规则网状的，Dipterygium glaucum 的纹饰是规则的，Gynandropsis gynandra 的纹饰是条纹规则的。基于花粉的整体特征，似乎该科的花粉学在属和种水平上都有帮助。
