College of Life Sciences, Shandong Agricultural University, Taian, China.
College of Forestry, Shandong Agricultural University, Taian, China.
J Appl Microbiol. 2019 Apr;126(4):1209-1220. doi: 10.1111/jam.14194. Epub 2019 Feb 18.
We aimed to develop a biological agent that regulates the microbial community structure of the poplar rhizosphere and alleviates the effects of continuous poplar cropping.
Poplar rhizosphere soils were treated with or without Paenibacillus polymyxa CP-S316 microbial fermentation medium. Real-time polymerase chain reaction was performed to measure bacteria and fungi in both groups, and microbial communities were analysed by metabarcoding. In fungi, the operational taxonomic units, abundance-based coverage estimator and Chao index of the CP-S316-treated group were significantly lower than those in the control check (CK) group. In bacteria, the proportions of Bacillus in the CP-S316 and CK groups were 5·20 and 2·38%, respectively, whereas those of Rhizoctonia were 2·20 and 5·82% respectively. The diameter at breast height, culturable bacteria and total bacteria of poplars treated with CP-S316 exceeded those in the CK group.
Our data confirmed that CP-S316 could improve the microbial community structure of poplar rhizosphere and promote the growth of poplars.
Research aimed at alleviating continuous cropping obstacles and promoting poplar growth via biocontrol agents is uncommon. We analysed the community structures of bacteria and fungi in rhizosphere soil to illustrate the use of CP-S316 for poplar cropping for improving plant health in the continuous cropping of poplar trees.
我们旨在开发一种生物制剂，调节杨树木根际微生物群落结构，缓解杨树连作的影响。
用或不用多粘类芽孢杆菌 CP-S316 微生物发酵培养基处理杨树根际土壤。实时聚合酶链反应用于测量两组细菌和真菌，并用代谢组学分析微生物群落。在真菌中，CP-S316 处理组的操作分类单位、基于丰度的覆盖估计和 Chao 指数明显低于对照（CK）组。在细菌中，CP-S316 和 CK 组的芽孢杆菌比例分别为 5.20%和 2.38%，而 Rhizoctonia 的比例分别为 2.20%和 5.82%。用 CP-S316 处理的杨树胸径、可培养细菌和总细菌均高于 CK 组。
我们的数据证实 CP-S316 可以改善杨树根际微生物群落结构，促进杨树生长。
旨在通过生物防治剂缓解连作障碍和促进杨树生长的研究并不常见。我们分析了根际土壤中细菌和真菌的群落结构，说明了 CP-S316 在杨树连作中用于改善植物健康，促进杨树的连续种植。
