Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju, Republic of Korea.
Crop Protection Division, National Institute of Agricultural Science, Wanju, Republic of Korea.
J Econ Entomol. 2019 Mar 21;112(2):835-841. doi: 10.1093/jee/toy404.
The two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), is one of the most important pest species, because it devastates many horticultural and ornamental crops and fruit trees. In the present study, we explored a field strain that was collected in January 2001 and then selected for 16 years for acequinocyl resistance. The resistance ratios calculated for the LC50 value in the laboratory-selected acequinocyl-resistant (LSAR16) strain was 4,237-fold higher than that of the susceptible strain. Pretreatment with the synergists piperonyl butoxide and S,S,S-tributyl-phosphorotrithioate significantly increased the toxicity of acequinocyl to the LSAR16 strain. Crossing experiments revealed that the resistance in the LSAR16 strain was maternally inherited, dominant, and monogenic. Furthermore, among individuals in the LSAR16 strain, 85.5-98.5% had the I256V mutation and 98-99% had the N321S mutation in mitochondrial cytochrome b. These results suggest that these two new point mutations contribute to acequinocyl resistance in T. urticae.
二斑叶螨，Tetranychus urticae Koch（蜱螨目：叶螨科），是最重要的害虫之一，因为它破坏了许多园艺和观赏作物和果树。在本研究中，我们探索了一个于 2001 年 1 月采集的田间种群，然后选择了 16 年用于抗性选育。在实验室选择的抗阿维菌素（LSAR16）菌株中，LC50 值的抗性比值比敏感菌株高 4237 倍。用增效剂胡椒基丁醚和 S，S，S-三丁基膦酸三硫酯预处理显著提高了阿维菌素对 LSAR16 菌株的毒性。杂交实验表明，LSAR16 菌株的抗性是母系遗传、显性和单基因的。此外，在 LSAR16 菌株的个体中，线粒体细胞色素 b 的 I256V 突变和 N321S 突变分别占 85.5-98.5%和 98-99%。这些结果表明，这两个新的点突变有助于二斑叶螨对阿维菌素的抗性。
