Environmental Sciences Branch, Canadian Nuclear Laboratories, Chalk River, Ontario, Canada.
Environ Toxicol Chem. 2019 Mar;38(3):585-590. doi: 10.1002/etc.4348. Epub 2019 Feb 11.
The objective of the present study was to establish a culture of Hyalella azteca that could be used for laboratory toxicity testing in low-ionic strength waters with electrical conductivities of <200 μS/cm. A wild strain of H. azteca was collected from Twin Lake, a small seepage lake with an electrical conductivity of 81 ± 27 μS/cm located on the property of Chalk River Laboratories in Chalk River, Canada. To determine the minimum aqueous ion requirements for an optimal culturing medium for the Twin Lake strain, Twin Lake was monitored for water quality and ionic content over 4 yr. Water quality parameters were averaged and used to formulate a medium containing NaHCO , CaCl , MgSO , KCl, NaBr, NaF, and LiCl, with an electrical conductivity of 89 ± 3 μS/cm. By evaluating survival and reproduction, it was concluded that this artificial medium promoted survival and supported reproduction (10 ± 4 neonates/female/wk) of the Twin Lake amphipod. The Twin Lake strain of H. azteca can, therefore, be maintained in laboratory settings, and this allows for toxicity testing to be conducted on low-ionic strength waters. Environ Toxicol Chem 2019;38:585-590. © 2019 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals, Inc. on behalf of SETAC.
本研究的目的是建立一种可以用于在电导率<200μS/cm 的低离子强度水中进行实验室毒性测试的食蚊鱼（Hyalella azteca）培养。从加拿大 chalk 河实验室产权的 Twin Lake 收集了一种野生食蚊鱼（H. azteca）菌株，这是一个电导率为 81±27μS/cm 的小型渗漏湖。为了确定 Twin Lake 菌株最佳培养介质的最低水离子要求，对 Twin Lake 的水质和离子含量进行了 4 年的监测。对水质参数进行了平均处理，并将其用于配制含有 NaHCO3、CaCl2、MgSO4、KCl、NaBr、NaF 和 LiCl 的培养基，电导率为 89±3μS/cm。通过评估存活率和繁殖力，得出结论认为这种人工培养基促进了 Twin Lake 桡足类动物的存活和繁殖（10±4 只幼体/雌体/周）。因此，可以在实验室环境中维持 Twin Lake 食蚊鱼（H. azteca）种群，这使得可以对低离子强度水进行毒性测试。环境毒理学与化学 2019；38:585-590。版权所有 © 2019 作者。环境毒理学与化学由 Wiley Periodicals, Inc. 代表 SETAC 出版。
