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使用基于光纤的荧光寿命成像技术对颈动脉生化成分进行无标记评估。

Label-free assessment of carotid artery biochemical composition using fiber-based fluorescence lifetime imaging.

作者信息

Alfonso-Garcia Alba, Haudenschild Anne K, Marcu Laura

机构信息

Department of Biomedical Engineering, University of California, Davis, 451 E. Health Sciences Dr., Davis, CA 95616, USA.

Authors contributed equally to this work.

出版信息

Biomed Opt Express. 2018 Aug 6;9(9):4064-4076. doi: 10.1364/BOE.9.004064. eCollection 2018 Sep 1.

DOI:10.1364/BOE.9.004064
PMID:30615748
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6157793/
Abstract

Novel diagnostic tools with the ability to monitor variations in biochemical composition and provide benchmark indicators of vascular tissue maturation are needed to create functional tissue replacements. We investigated the ability of fiber-based, label-free multispectral fluorescent lifetime imaging (FLIm) to quantify the anatomical variations in biochemical composition of native carotid arteries and validated these results against biochemical assays. FLIm-derived parameters in spectral band 415-455 nm correlated with tissue collagen content () and cell number () and in spectral band 465-553 nm strongly correlated with elastin content (). These results suggest that FLIm holds great potential for assessing vascular tissue maturation and functional properties based on tissue autofluorescence.

摘要

为了制造功能性组织替代物，需要具备监测生化组成变化并提供血管组织成熟度基准指标能力的新型诊断工具。我们研究了基于光纤的无标记多光谱荧光寿命成像（FLIm）量化天然颈动脉生化组成解剖学变化的能力，并通过生化分析验证了这些结果。415 - 455 nm光谱带中FLIm衍生参数与组织胶原蛋白含量（）和细胞数量（）相关，而在465 - 553 nm光谱带中与弹性蛋白含量（）高度相关。这些结果表明，FLIm在基于组织自发荧光评估血管组织成熟度和功能特性方面具有巨大潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e99a/6157793/cf6ad96ad6b9/boe-9-9-4064-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e99a/6157793/72f29f22e233/boe-9-9-4064-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e99a/6157793/b98835acd75d/boe-9-9-4064-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e99a/6157793/40ecc11b1d21/boe-9-9-4064-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e99a/6157793/80e58cf34d69/boe-9-9-4064-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e99a/6157793/cf6ad96ad6b9/boe-9-9-4064-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e99a/6157793/72f29f22e233/boe-9-9-4064-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e99a/6157793/b98835acd75d/boe-9-9-4064-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e99a/6157793/40ecc11b1d21/boe-9-9-4064-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e99a/6157793/80e58cf34d69/boe-9-9-4064-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e99a/6157793/cf6ad96ad6b9/boe-9-9-4064-g005.jpg

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