Department of Human Health and Nutritional Science, University of Guelph, 50 Stone Road East, Guelph, ON N1G 2W1, Canada.
Department of Clinical Studies, Ontario Veterinary College, University of Guelph, 50 McGilvray Lane, Guelph, ON N1G 2W1, Canada.
Exp Gerontol. 2019 Apr;118:31-38. doi: 10.1016/j.exger.2019.01.002. Epub 2019 Jan 4.
This study aimed to investigate the association between naturally occurring spinal osteoarthritis (OA) (L3-L5), the expression of substance P (SP) centrally (L4-L5) and the presence of neurogenic inflammation within the neurosegmentally linked quadriceps (L2-L5) in elderly rats versus young controls.
Eight aged (27 ± 3.2 months) and six young (4 ± 0.0 months) male Wistar Kyoto rats were euthanized and submitted to micro-computerized tomography for determination of spine OA. SP expression (% area) at the dorsal horn of the spinal cord as well as the relative expression of SP and protease-activated receptor 2 (PAR2) to alpha-tubulin within quadriceps muscle were determined by immunohistochemistry and Western Blot.
Spine osteoarthritis was confirmed in all aged rats but no young controls. Aged rats expressed significant increase of SP protein expression within the dorsal horn (MD = 0.086; 95% CI [0.026 to 0.145]; p = 0.0094) and quadriceps (MD = 1.209; 95% CI [0.239 to 2.179]; p = 0.0191) and PAR2 (MD = 0.797; 95% CI [0.160 to 1.435]; p = 0.0187) compared to young controls.
These observations provide novel insight into the potential role of neurogenic inflammation in the pathophysiology of myofascial pain syndrome in the naturally occurring spinal OA in elderly population.
本研究旨在探讨老年大鼠与年轻对照组相比，脊柱自然发生的骨关节炎（OA）（L3-L5）、中央物质 P（SP）的表达（L4-L5）以及与神经节段相关的股四头肌（L2-L5）内神经源性炎症的存在之间的关系。
8 只老年（27±3.2 个月）和 6 只年轻（4±0.0 个月）雄性 Wistar Kyoto 大鼠被安乐死，并进行微计算机断层扫描以确定脊柱 OA。通过免疫组织化学和 Western Blot 测定脊髓背角中 SP 表达（%面积）以及股四头肌中 SP 和蛋白酶激活受体 2（PAR2）与微管蛋白的相对表达。
所有老年大鼠均证实存在脊柱骨关节炎，但年轻对照组没有。老年大鼠的背角（MD=0.086；95%CI[0.026 至 0.145]；p=0.0094）和股四头肌（MD=1.209；95%CI[0.239 至 2.179]；p=0.0191）中的 SP 蛋白表达显著增加，PAR2 （MD=0.797；95%CI[0.160 至 1.435]；p=0.0187）与年轻对照组相比。
这些观察结果为神经源性炎症在老年人群中自然发生的脊柱 OA 肌筋膜疼痛综合征的病理生理学中的潜在作用提供了新的见解。
