Human Health and Nutritional Science, University of Guelph, Guelph, Ontario N1G2W1, Canada; Division of Research and Innovation, Canadian Memorial Chiropractic College, Toronto-ON, Canada.
Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G2W1, Canada.
Exp Gerontol. 2021 Jul 1;149:111311. doi: 10.1016/j.exger.2021.111311. Epub 2021 Mar 17.
Naturally occurring spine osteoarthritis is clinically associated with the manifestation of chronic inflammatory muscle (myofascial) disease. The purpose of this study was to investigate the causal association between experimentally induced spine osteoarthritis and neurogenic inflammatory responses within neurosegmentally linked myotomes. Wistar Kyoto rats were randomly assigned to spine facet compression surgery (L4-L6) or sham surgery. Animals exposed to facet compression surgery demonstrated radiographic signs of facet-osteoarthritis (L4-L6 spinal levels) and sensory changes (allodynia, thermal hyperalgesia) at 7, 14 and 21 days post-intervention, consistent with the induction of central sensitization; no radiologic or sensory changes were observed after sham surgery. Increased levels of proinflammatory biomarkers including substance P (SP), calcitonin gene related peptide (CGRP), protease-activated receptor-2 (PAR2) and calcium/calmodulin dependent protein kinase II (CaMKII) were observed post-surgery within neurosegmentally-linked rectus femoris (L2-L5) muscle when compared to the non-segmentally linked biceps brachii (C4-C7) muscle; no differences were observed between muscles in the sham surgery group. These findings offer novel insight into the potential role of spine osteoarthritis and neurogenic inflammatory mechanisms in the pathophysiology of chronic inflammatory muscle (myofascial) disease.
自发性脊柱骨关节炎在临床上与慢性炎症性肌肉（肌筋膜）疾病的表现有关。本研究旨在探讨实验性脊柱骨关节炎与神经节段相关肌节内神经源性炎症反应之间的因果关系。Wistar Kyoto 大鼠被随机分配接受脊柱小关节压迫手术（L4-L6）或假手术。暴露于小关节压迫手术的动物在干预后 7、14 和 21 天表现出小关节骨关节炎的放射学迹象（L4-L6 脊柱水平）和感觉变化（感觉过敏、热痛觉过敏），与中枢敏化的诱导一致；假手术后未观察到放射学或感觉变化。与非节段相关的肱二头肌（C4-C7）肌肉相比，手术后在神经节段相关的股直肌（L2-L5）肌肉中观察到促炎生物标志物水平升高，包括 P 物质（SP）、降钙素基因相关肽（CGRP）、蛋白酶激活受体-2（PAR2）和钙/钙调蛋白依赖性蛋白激酶 II（CaMKII）；假手术组肌肉之间没有差异。这些发现为脊柱骨关节炎和神经源性炎症机制在慢性炎症性肌肉（肌筋膜）疾病的病理生理学中的潜在作用提供了新的见解。
