Bellinger Y, Larher F
Laboratoire de Biologie végétale, Université de Rennes 1, France.
Can J Microbiol. 1988 May;34(5):605-12. doi: 10.1139/m88-100.
Glycerol, arabitol and trehalose were the principle solutes detected in cellular extracts of Hansenula anomala, using natural-abundance 13C nuclear magnetic resonance spectroscopy. Only the two polyols accumulated in response to increased salinity, glycerol increase being far greater. Arabitol content also increased with culture age, independently of the presence or absence of salt and in line with the evolution of trehalose content. Glycerol retention potential was 15 times greater for Hansenula than for Saccharomyces cerevisiae. The former displayed the specific property of increasing this capacity in high salt concentrations. Under such conditions its growth was associated with a limited increase in glucose consumption per unit biomass, relative to S. cerevisiae, the salt-sensitive reference yeast. In addition, a polysaccharide, the chemical nature of which was not further characterized, was detected exclusively in the external medium of Hansenula growing in the presence of salt.
使用天然丰度的¹³C核磁共振光谱法，在异常汉逊酵母的细胞提取物中检测到甘油、阿拉伯糖醇和海藻糖是主要溶质。只有这两种多元醇会随着盐度增加而积累，甘油的增加幅度要大得多。阿拉伯糖醇含量也随着培养时间增加，与盐的存在与否无关，且与海藻糖含量的变化一致。异常汉逊酵母的甘油保留潜力比酿酒酵母大15倍。前者具有在高盐浓度下增加这种能力的特殊性质。在这种条件下，相对于对盐敏感的对照酵母酿酒酵母，其生长与单位生物量葡萄糖消耗的有限增加有关。此外，在有盐存在的情况下生长的异常汉逊酵母的外部培养基中专门检测到一种多糖，其化学性质未进一步表征。
