Miao P Z, Yang Y, Chen E B, Zhu G Q, Wang B, Dai Z
Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai 200030, China.
Zhonghua Gan Zang Bing Za Zhi. 2018 Nov 20;26(11):842-846. doi: 10.3760/cma.j.issn.1007-3418.2018.11.007.
To investigates the role of piwi-interacting RNAs (piRNA) in the occurrence and development of hepatocellular carcinoma. Second-generation small RNA sequencing was performed on cancer and paracancerous tissues, metastatic and non-metastatic liver cancer tissues of patients with liver cancer, and the sequencing data were filtered out for the common RNA sequences to be repeated. The piRNA predictor was used to forecast the possible new piRNA merged with the downloaded known piRNA to screen out distinction. A miRanda algorithm was used to predict the corresponding target genes and functional enrichment analysis. piRNA was selected for experimental functional (migration) analysis. An independent t-test was used to compare means between the two groups. 66 772 piRNAs (known 149) were obtained by sequencing. 241 piRNAs were found in cancer and paracancerous tissues, and 1 634 piRNAs were found in metastatic and non-metastatic tumors. Analysis of the GO and KEGG pathways of the target genes of differential piRNAs revealed that they were mainly involved in cell adhesion. An experimental functional analysis was performed on the selected Pirna (PIR1/97), which showed that it promoted the migration of hepatoma cells (LM3: = 8.829, < 0.05; PLC: = 7.318, < 0.05). The expression levels of piRNA in hepatocellular carcinoma patients with cancer and paracancerous tissues, metastasis and non-metastatic liver cancer tissues are different and it could be entailed in the metastasis process of hepatocellular carcinoma. Hence, experimental functional analysis is required for research and experimental confirmation.
为研究与Piwi相互作用的RNA（piRNA）在肝细胞癌发生发展中的作用。对肝癌患者的癌组织和癌旁组织、转移性和非转移性肝癌组织进行了第二代小RNA测序，并对测序数据进行过滤以获取常见的重复RNA序列。使用piRNA预测器预测可能与下载的已知piRNA合并的新piRNA，以筛选出差异。使用miRanda算法预测相应的靶基因并进行功能富集分析。选择piRNA进行实验性功能（迁移）分析。采用独立样本t检验比较两组均值。通过测序获得了66772个piRNA（已知149个）。在癌组织和癌旁组织中发现了241个piRNA，在转移性和非转移性肿瘤中发现了1634个piRNA。对差异piRNA靶基因的GO和KEGG通路分析表明，它们主要参与细胞黏附。对所选的Pirna（PIR1/97）进行了实验性功能分析，结果表明它促进了肝癌细胞的迁移（LM3：t = 8.829，P < 0.05；PLC：t = 7.318，P < 0.05）。piRNA在肝癌患者的癌组织和癌旁组织、转移性和非转移性肝癌组织中的表达水平不同，可能参与了肝细胞癌的转移过程。因此，需要进行实验性功能分析以进行研究和实验验证。
