Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University, 17. Listopadu 12, 771 46, Olomouc, Czech Republic.
Department of Botany, Faculty of Science, Palacký University, Šlechtitelů 27, 783 71, Olomouc, Czech Republic.
Anal Bioanal Chem. 2019 Feb;411(6):1169-1180. doi: 10.1007/s00216-018-1551-3. Epub 2019 Jan 7.
Atmospheric solids analysis probe mass spectrometry (ASAP-MS) was used for the first time for direct surface analysis of plant material. It can be readily used for surface analysis of whole and intact pea seeds and their seed coats, and for the study of the profile of fatty acids on the outer surface. Furthermore, ASAP-MS in combination with multivariate statistics allowed classification of pea genotypes with respect to physical dormancy and investigation of related biological markers. Hexacosanoic and octacosanoic acids were suggested to be important markers likely influencing water transport through the seed coat into the embryo (with the highest significance for dormant L100 genotype). ASAP-MS provided higher selectivity and better signal of fatty acids compared to (MA)LDI-MS (laser desorption ionization mass spectrometry either matrix free or matrix assisted) providing on the other hand spatial distribution information and results obtained by both methods are mutually supportive. The developed ASAP-MS method and obtained results can be widely utilized in biological, food, and agricultural research. Graphical abstract ᅟ.
大气固体分析探针质谱（ASAP-MS）首次被用于直接分析植物材料的表面。它可以很容易地用于分析整个完整的豌豆种子及其种皮的表面，并研究脂肪酸在外层表面的分布情况。此外，ASAP-MS 与多元统计相结合，允许根据物理休眠对豌豆基因型进行分类，并研究相关的生物标志物。二十六烷酸和二十八烷酸被认为是可能影响水通过种皮进入胚的重要标志物（对休眠 L100 基因型的影响最大）。与 MALDI-MS（基质辅助激光解吸电离质谱）相比，ASAP-MS 提供了更高的脂肪酸选择性和更好的信号（后者提供了空间分布信息），而另一方面，两种方法的结果是相互支持的。所开发的 ASAP-MS 方法和获得的结果可以广泛应用于生物、食品和农业研究。
