Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University, 17. Listopadu 12, 771 46 Olomouc, Czech Republic.
Department of Botany, Faculty of Science, Palacký University, Šlechtitelů 27, 783 71 Olomouc, Czech Republic.
Int J Mol Sci. 2017 Oct 21;18(10):2196. doi: 10.3390/ijms18102196.
Seed coats of six pea genotypes contrasting in dormancy were studied by laser desorption/ionization mass spectrometry (LDI-MS). Multivariate statistical analysis discriminated dormant and non-dormant seeds in mature dry state. Separation between dormant and non-dormant types was observed despite important markers of particular dormant genotypes differ from each other. Normalized signals of long-chain hydroxylated fatty acids (HLFA) in dormant JI64 genotype seed coats were significantly higher than in other genotypes. These compounds seem to be important markers likely influencing JI64 seed imbibition and germination. HLFA importance was supported by study of recombinant inbred lines (JI64xJI92) contrasting in dormancy but similar in other seed properties. Furthemore HLFA distribution in seed coat was studied by mass spectrometry imaging. HLFA contents in strophiole and hilum are significantly lower compared to other parts indicating their role in water uptake. Results from LDI-MS experiments are useful in understanding (physical) dormancy (first phases of germination) mechanism and properties related to food processing technologies (e.g., seed treatment by cooking).
六种豌豆基因型的种皮在休眠方面存在差异，通过激光解吸/电离质谱（LDI-MS）对其进行了研究。多变量统计分析可区分成熟干燥状态下休眠和非休眠种子。尽管特定休眠基因型的重要标记彼此不同，但仍观察到休眠和非休眠类型之间的分离。休眠型 JI64 基因型种皮中长链羟基化脂肪酸（HLFA）的归一化信号明显高于其他基因型。这些化合物似乎是重要的标记物，可能影响 JI64 种子的吸胀和萌发。HLFA 的重要性通过在休眠方面存在差异但在其他种子特性方面相似的重组自交系（JI64xJI92）研究得到了支持。此外，还通过质谱成像研究了种皮中 HLFA 的分布。与其他部分相比，珠柄和种脐中的 HLFA 含量明显较低，表明它们在吸水过程中的作用。LDI-MS 实验的结果有助于理解（物理）休眠（萌发的第一阶段）机制以及与食品加工技术相关的特性（例如，通过烹饪对种子进行处理）。
