大肠杆菌中的信使核糖核酸识别：与16S核糖体核糖核酸相互作用的一个可能的第二位点。
Messenger RNA recognition in Escherichia coli: a possible second site of interaction with 16S ribosomal RNA.
作者信息
Petersen G B, Stockwell P A, Hill D F
机构信息
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
出版信息
EMBO J. 1988 Dec 1;7(12):3957-62. doi: 10.1002/j.1460-2075.1988.tb03282.x.
Abstract
Examination of the nucleotides following the ATG or GTG initiation codons of a file of 251 genes from Escherichia coli has shown that 247 (98.4%) of them contain a sequence of at least three and 168 (66.9%) of them a sequence of at least four consecutive nucleotides that is complementary to some part of the 16 nt at the 5' terminus of the bacterial 16S rRNA. It is proposed that this sequence, which falls within the first 24 nt coding for the genetic message, might be involved in mRNA recognition through a mechanism analogous to the well-established 'Shine--Dalgarno' interaction with the 3' terminus of the 16S rRNA. Comparison of these data with data derived from a file of 117 'false' gene starts that have a Shine--Dalgarno-like sequence followed by a suitably spaced ATG or GTG triplet but which are believed not to lie at the beginnings of genetic messages shows the association that we have found to be statistically significant at the 99.9% level.
摘要
对来自大肠杆菌的251个基因的文件中ATG或GTG起始密码子后的核苷酸进行检查发现，其中247个（98.4%）含有至少三个连续核苷酸的序列，168个（66.9%）含有至少四个连续核苷酸的序列，该序列与细菌16S rRNA 5'末端的16个核苷酸的某些部分互补。有人提出，这个位于编码遗传信息的前24个核苷酸内的序列，可能通过一种类似于已确立的与16S rRNA 3'末端的“Shine-Dalgarno”相互作用的机制参与mRNA识别。将这些数据与来自117个“假”基因起始位点的文件数据进行比较，这些“假”基因起始位点有一个类似Shine-Dalgarno的序列，后面跟着一个间隔合适的ATG或GTG三联体，但据信它们并不位于遗传信息的起始位置，结果表明我们发现的这种关联在99.9%的水平上具有统计学意义。
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本文引用的文献
1
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Nucleic Acids Res. 1982 May 11;10(9):2997-3011. doi: 10.1093/nar/10.9.2997.
2
Characterization of translational initiation sites in E. coli.大肠杆菌中转录起始位点的表征
Nucleic Acids Res. 1982 May 11;10(9):2971-96. doi: 10.1093/nar/10.9.2971.
3
The ribosome binding sites recognized by E. coli ribosomes have regions with signal character in both the leader and protein coding segments.大肠杆菌核糖体识别的核糖体结合位点在前导序列和蛋白质编码序列中都有具有信号特征的区域。
Nucleic Acids Res. 1980 Sep 11;8(17):3895-907. doi: 10.1093/nar/8.17.3895.
4
lacZ translation initiation mutations.β-半乳糖苷酶翻译起始突变
J Mol Biol. 1984 Aug 25;177(4):663-83. doi: 10.1016/0022-2836(84)90043-3.
5
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J Mol Biol. 1982 Dec 25;162(4):729-73. doi: 10.1016/0022-2836(82)90546-0.
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7
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