Department of Plant and Environmental Protection, PARC Institute of Advanced Studies in Agriculture (Affiliated with Quaid-i-Azam University), National Agricultural Research Centre, Islamabad, 45500, Pakistan.
Laboratory of Fungal Interaction and Molecular Biology (donated by IFO), Department of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan.
J Gen Virol. 2023 Nov;104(11). doi: 10.1099/jgv.0.001925.
is a thermo-tolerant, ubiquitous fungus commonly found in food products, indoor environments, soil and clinical samples. It is a well-known biocontrol agent used against phytopathogenic fungi and its metabolites have many industrial applications. Rare reports of related human infections have been found in the medical literature. In this study, we report for the first time the infection of isolated from a soil sample collected in a rice field with a double-stranded RNA virus, Paeciliomyces variotii partitivirus 1 (PvPV-1) in the family harboured icosahedral virus particles 30 nm in diameter with two dsRNA segments 1758 and 1356 bp long. Both dsRNA1 and dsRNA2 have a single open reading frame encoding proteins of 63 and 40 kDa, respectively. These proteins have significant similarity to the RNA-dependent RNA polymerase and capsid protein encoded by the genomic segments of several viruses from the family . Phylogenetic analysis revealed that PvPV-1 belongs to the family but in an unclassified group/genus, tentatively nominated Zetapartitivirus. PvPV-1 was found to increase the growth rate of the host fungus, as indicated by time course experiments performed on a range of different media for virus-infected and virus-free isogenic lines. Further, dual-culture assays performed for both isogenic lines confirmed the antagonistic potential of against other phytopathogenic fungi. The findings of this study assist us in understanding as a potential biocontrol agent, together with plant-fungus-virus interactions.
是一种耐热、无处不在的真菌，通常存在于食品、室内环境、土壤和临床样本中。它是一种众所周知的生防真菌，其代谢产物在许多工业中有应用。在医学文献中很少有相关人类感染的报道。在这项研究中，我们首次报告了从稻田土壤样本中分离出的一株 ，该菌携带双链 RNA 病毒，即拟青霉节段病毒 1（PvPV-1），属于 科，含有直径为 30nm 的二十面体病毒粒子，两个 dsRNA 片段长 1758 和 1356bp。dsRNA1 和 dsRNA2 都有一个单一的开放阅读框，分别编码 63 和 40kDa 的蛋白质。这些蛋白质与家族中几个病毒的基因组片段编码的 RNA 依赖性 RNA 聚合酶和衣壳蛋白具有显著的相似性。系统发育分析表明，PvPV-1 属于 科，但在一个未分类的组/属中，暂命名为 Zetapartitivirus。时间进程实验表明，PvPV-1 能提高宿主真菌的生长速度，实验在一系列不同的培养基上对感染病毒和未感染病毒的同基因系进行。此外，对两种同基因系进行的双培养试验证实了 对其他植物病原真菌的拮抗潜力。本研究的结果有助于我们了解 作为一种潜在的生防剂，以及植物-真菌-病毒的相互作用。
