Department of Restorative Dentistry, Araçatuba Dental School, UNESP-Univ Estadual Paulista, Araçatuba, São Paulo, Brazil.
Department of Basic Sciences, Araçatuba Dental School, UNESP-Univ Estadual Paulista, Araçatuba, São Paulo, Brazil.
PLoS One. 2019 Jan 8;14(1):e0210338. doi: 10.1371/journal.pone.0210338. eCollection 2019.
This study aimed to evaluate neuropeptide expression after bleaching treatment using histopathological and immunohistochemical analyses and the effects of hydrocortisone and acetaminophen on pulp inflammation, sine dental bleaching and inflammation first occur, and only then, the treatmentt. Sixty-three rats were divided into three groups (n = 21) according to the pain-relieving therapy used: I-control; II-topical application of Otosporin for 10 min after the bleaching treatment; III-oral administration of paracetamol 30 min before whitening and then every 12h. In all the study groups, placebo gel was applied to the left upper jaw (control) and a 35% H2O2-based whitening gel was applied to the right upper jaw for 45 min. Seven animals from each group were euthanized at different time points: 0h after treatment, 24h, and 48h. After euthanasia, the first molar on each side was analyzed by histology and immunohistochemistry to assess the degree of inflammation and verify the presence of the neuropeptides, substance P (SP) and calcitonin gene-related peptide (CGRP). The data were analyzed using the statistical nonparametric Kruskal-Wallis test followed by Dunn's test for individual comparisons. Extensive areas of necrosis were observed in the groups that received bleaching treatment only, whereas reduced damage were obtained in the group treated with Otosporin. The immunohistochemical analysis showed positive immunolabeling in all groups, including the control, but this was stronger in the groups that received bleaching treatment. The best results were obtained in the group that received treatment with Otosporin. The use of Otosporin after dental bleaching minimized the side effects of this treatment.
本研究旨在通过组织病理学和免疫组织化学分析评估漂白治疗后的神经肽表达，并研究氢化可的松和对乙酰氨基酚对牙髓炎症、无牙漂白和炎症的影响，只有在这种情况下，才需要进行治疗。63 只大鼠根据使用的止痛治疗方法分为三组（n = 21）：I-对照组；II-漂白治疗后局部应用 Otosporin 10 分钟；III-漂白前 30 分钟口服扑热息痛，然后每 12 小时一次。在所有研究组中，左侧上颌（对照）应用安慰剂凝胶，右侧上颌应用 35%H2O2 基漂白凝胶 45 分钟。每组有 7 只动物在不同时间点处死：治疗后 0 小时、24 小时和 48 小时。安乐死后，对每侧第一磨牙进行组织学和免疫组织化学分析，以评估炎症程度并验证神经肽物质 P（SP）和降钙素基因相关肽（CGRP）的存在。使用非参数统计 Kruskal-Wallis 检验分析数据，然后使用 Dunn 检验进行个体比较。仅接受漂白治疗的组观察到广泛的坏死区，而接受 Otosporin 治疗的组损伤减少。免疫组织化学分析显示所有组均有阳性免疫标记，包括对照组，但接受漂白治疗的组更强。接受 Otosporin 治疗的组效果最佳。在牙齿漂白后使用 Otosporin 可以最大程度地减少这种治疗的副作用。
