采用超高效液相色谱/三重四极杆串联质谱法同时评价附子对大鼠口服给药后三种二酯型生物碱药代动力学的影响

Simultaneous Evaluation of the Influence of on the Pharmacokinetics of Three Diester Alkaloids after Oral Administration of Aconiti Lateralis Radix in Rats Using UHPLC/QQQ-MS/MS.

作者信息

Yang Liang, Wang Yuguang, Huang Guangyao, Li Jian, Zhang Zhaoyan, Ma Zengchun, Gao Yue

机构信息

Beijing Institution of Radiation Medicine, Beijing 100850, China.

出版信息

Evid Based Complement Alternat Med. 2018 Dec 9;2018:6527549. doi: 10.1155/2018/6527549. eCollection 2018.

OBJECTIVES

To investigate whether () could affect the metabolism of Diester Alkaloids (DAs) derived from Aconiti Lateralis Radix in vivo.

METHODS AND RESULTS

24 male Sprague-Dawley rats were randomized for 7-day treatment with (low, middle, and high), or vehicle. Aconiti Lateralis Radix was administered orally to each group on the 8th day. Plasma samples were collected, and Xevo TQ-S was used to detect the concentration of aconitine, mesaconitine, and hypaconitine in plasma. We describe a fast and reproducible method to detect the concentration of aconitine, mesaconitine, and hypaconitine in plasma. Compared to the control group, the AUC of three DAs increased in both the middle and high dosing groups. The Vz/F of three DAs in these groups as well as the CLz/F of aconitine in all groups and the CLz/F of mesaconitine and hypaconitine in middle and high groups were decreased compared to the control group.

CONCLUSION

Orally administrated potentially inhibits the metabolism of DAs from Aconiti Lateralis Radix in rats.

目的

研究（）是否会影响附子中双酯型生物碱（DAs）在体内的代谢。

方法与结果

将24只雄性Sprague-Dawley大鼠随机分为3组，分别用（低、中、高剂量）或赋形剂进行为期7天的治疗。第8天对每组大鼠口服附子。采集血浆样本，采用Xevo TQ-S检测血浆中乌头碱、中乌头碱和次乌头碱的浓度。我们描述了一种快速且可重复的方法来检测血浆中乌头碱、中乌头碱和次乌头碱的浓度。与对照组相比，中、高剂量组三种双酯型生物碱的AUC均升高。与对照组相比，这些组中三种双酯型生物碱的Vz/F以及所有组中乌头碱的CLz/F和中、高剂量组中中乌头碱和次乌头碱的CLz/F均降低。