Wang Jia, Lin Weijun, Yin Zhongdong, Wang Libing, Dong ShuBin, An Jiyong, Lin Zixin, Yu Haiyan, Shi Lingling, Lin Shanzhi, Chen Shaoliang
1Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Biotechnology, School of Soil and Water Conservation, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, 100083 China.
2Institute of Botany, Chinese Academy of Sciences, Beijing, 100093 China.
Biotechnol Biofuels. 2019 Jan 4;12:6. doi: 10.1186/s13068-018-1347-x. eCollection 2019.
Based on our previous studies of 17 germplasms, one plus tree with high quality and quantity of seed oils has emerged as novel potential source of biodiesel. To better develop seed oils as woody biodiesel, a concurrent exploration of oil content, FA composition, biodiesel yield and fuel properties as well as prediction model construction for fuel properties was conducted on developing seeds to determine the optimal seed harvest time for producing high-quality biodiesel. Oil synthesis required supply of carbon source, energy and FA, but their transport mechanisms still remains enigmatic. Our recent 454 sequencing of could provide long-read sequences to identify membrane transporters for a better understanding of regulatory mechanism for high oil production in developing seeds.
To better develop the seed oils of as woody biodiesel, we firstly focused on a temporal and comparative evaluation of growth tendency, oil content, FA composition, biodiesel yield and fuel properties as well as model construction for biodiesel property prediction in different developing seeds from plus tree (accession AS-80), revealing that the oils from developing seeds harvested after 60 days after flowering (DAF) could be as novel potential feedstock for producing biodiesel with ideal fuel property. To gain new insight into membrane transport mechanism for high oil yield in developing seeds of , we presented a global analysis of transporter based on our recent 454 sequencing data of . We annotated a total of 116 genes for membrane-localized transporters at different organelles (plastid, endoplasmatic reticulum, tonoplast, mitochondria and peroxisome), of which some specific transporters were identified to be involved in carbon allocation, metabolite transport and energy supply for oil synthesis by both RT-PCR and qRT-PCR. Importantly, the transporter-mediated model was well established for high oil synthesis in developing seeds. Our findings could help to reveal molecular mechanism of increased oil production and may also present strategies for engineering oil accumulation in oilseed plants.
This study presents a temporal and comparative evaluation of developing seed oils as a potential feedstock for producing high-quality biodiesel and a global identification for membrane transporters was to gain better insights into regulatory mechanism of high oil production in developing seeds of . Our findings may present strategies for developing woody biodiesel resources and engineering oil accumulation.
基于我们之前对17种种质的研究，一株种子油质量和产量都很高的优树已成为生物柴油的新型潜在来源。为了更好地将种子油开发为木质生物柴油，我们对发育中的种子进行了油含量、脂肪酸组成、生物柴油产量和燃料特性的同步探索，并构建了燃料特性预测模型，以确定生产高质量生物柴油的最佳种子收获时间。油脂合成需要碳源、能量和脂肪酸的供应，但其运输机制仍然不明。我们最近对[物种名称]进行的454测序能够提供长读长序列，以鉴定膜转运蛋白，从而更好地理解发育中种子高产油的调控机制。
为了更好地将[物种名称]的种子油开发为木质生物柴油，我们首先对优树（种质编号AS - 80）不同发育阶段种子的生长趋势、油含量、脂肪酸组成、生物柴油产量和燃料特性进行了时间和比较评估，并构建了生物柴油特性预测模型，结果表明，开花后60天（DAF）收获的发育中种子油可作为生产具有理想燃料特性生物柴油的新型潜在原料。为了深入了解[物种名称]发育中种子高产油的膜转运机制，我们基于最近对[物种名称]的454测序数据对转运蛋白进行了全面分析。我们总共注释了116个位于不同细胞器（质体、内质网、液泡膜、线粒体和过氧化物酶体）的膜定位转运蛋白基因，通过RT - PCR和qRT - PCR鉴定出其中一些特定转运蛋白参与了油脂合成的碳分配、代谢物运输和能量供应。重要的是，建立了转运蛋白介导的[物种名称]发育中种子高产油模型。我们的研究结果有助于揭示产油量增加的分子机制，也可能为油料植物中油脂积累工程提供策略。
本研究对[物种名称]发育中种子油作为生产高质量生物柴油的潜在原料进行了时间和比较评估，并对膜转运蛋白进行了全面鉴定，以更好地了解[物种名称]发育中种子高产油的调控机制。我们的研究结果可能为开发木质生物柴油资源和油脂积累工程提供策略。
