Espinosa Omar Ariel, Benevides Ferreira Silvana Margarida, Longhi Palacio Fabiana Gulin, Cortela Denise da Costa Boamorte, Ignotti Eliane
Post Graduation Program in Health Science, Faculty of Medicine, Federal University of Mato Grosso (UFMT), Cuiaba, Mato Grosso, Brazil.
Department of Medicine, Faculty of Health Sciences, State University of Mato Grosso (UNEMAT), Caceres, Mato Grosso, Brazil.
Can J Infect Dis Med Microbiol. 2018 Nov 25;2018:9828023. doi: 10.1155/2018/9828023. eCollection 2018.
IgM against may be detected by enzyme-linked immunosorbent assays (ELISAs) based on phenolic glycolipid I (PGL-I) or natural disaccharide octyl bovine serum albumin (ND-O-BSA) as antigens, and the IgG response can be detected by an ELISA based on lipid droplet protein 1 (LID-1). The titers of antibodies against these antigens vary with operational classification. The aim of this study was to compare the accuracy of ELISAs involving PGL-I and ND-O-BSA with that involving LID-1. We included studies that analyze multibacillary and paucibacillary leprosy cases and evaluate the diagnostic accuracy of ELISAs based on LID-1 and/or PGL-I or ND-O-BSA as antigens to measure antibody titers against . Studies were found via PubMed, the Virtual Health Library Regional Portal, Literatura Latino-Americana e do Caribe em Ciências da Saúde, Índice Bibliográfico Espanhol de Ciências de Saúde, the Brazilian Society of Dermatology, National Institute for Health and Clinical Excellence, Cochrane Library, Embase (the Elsevier database), and Cumulative Index to Nursing and Allied Health Literature. The Quality Assessment of Diagnostic Accuracy Studies served as a methodological validity tool. Quantitative data were extracted using the Standards for Reporting of Diagnostic Accuracy. Sensitivity, specificity, and a diagnostic odds ratio were calculated, and a hierarchical summary receiver-operating characteristic curve and forest plots were constructed. The protocol register code for this meta-analysis is PROSPERO 2017: CRD42017055983. Nineteen studies were included. ND-O-BSA showed better overall performance in terms of sensitivity, specificity, positive and negative likelihood ratios, and diagnostic odds ratio when compared with PGL-I and LID-1. The multibacillary group showed better performance on these parameters (than the paucibacillary group did), at 94%, 99%, 129, 0.05, and 2293, respectively. LID-1 did not provide any advantage regarding the overall estimate of sensitivity in comparison with PGL-I or ND-O-BSA.
基于酚糖脂I（PGL-I）或天然二糖辛基牛血清白蛋白（ND-O-BSA）作为抗原的酶联免疫吸附测定（ELISA）可检测出针对……的IgM，而基于脂滴蛋白1（LID-1）的ELISA可检测出IgG反应。针对这些抗原的抗体滴度因操作分类而异。本研究的目的是比较涉及PGL-I和ND-O-BSA的ELISA与涉及LID-1的ELISA的准确性。我们纳入了分析多菌型和少菌型麻风病例的研究，并评估基于LID-1和/或PGL-I或ND-O-BSA作为抗原的ELISA在测量针对……的抗体滴度时的诊断准确性。通过PubMed、虚拟健康图书馆区域门户、拉丁美洲和加勒比卫生科学文献数据库、西班牙卫生科学文献索引、巴西皮肤病学会、国家卫生与临床优化研究所、考克兰图书馆、Embase（爱思唯尔数据库）以及护理及相关健康文献累积索引找到相关研究。诊断准确性研究的质量评估用作方法学有效性工具。使用诊断准确性报告标准提取定量数据。计算敏感性、特异性和诊断比值比，并构建分层汇总接受者操作特征曲线和森林图。该荟萃分析的方案注册代码为PROSPERO 2017：CRD42017055983。纳入了19项研究。与PGL-I和LID-1相比，ND-O-BSA在敏感性、特异性、阳性和阴性似然比以及诊断比值比方面表现出更好的总体性能。多菌型组在这些参数上表现更好（优于少菌型组），分别为94%、99%、129、0.05和2293。与PGL-I或ND-O-BSA相比，LID-1在敏感性的总体估计方面没有任何优势。
