Hessami Mohammad Javad, Cheng Sit Foon, Ambati Ranga Rao, Yin Yeong Hui, Phang Siew Moi
1Institute of Biological Sciences, Faculty of Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia.
2Department of Chemistry, Faculty of Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia.
3 Biotech. 2019 Jan;9(1):25. doi: 10.1007/s13205-018-1549-8. Epub 2019 Jan 2.
In this study, is investigated for ethanol production. A combination of factors including different temperatures, acid concentration and incubation time was evaluated to determine the suitable saccharification conditions. The combination of 2.5% (w/v) HSO at 120 °C for 40 min was selected for hydrolysis of the seaweed biomass, followed by purification, and fermentation to yield ethanol. The galactose and glucose were dominant reducing sugars in the hydrolysate and under optimum condition of dilute acid hydrolysis, 39.42% of reducing sugars was produced and fermentation resulted in ethanol concentration of 13.27 ± 0.47 g/L. A modified method was evaluated for sample preparation for gas chromatography (GC) analysis of the ethanol content. A solvent mixture of acetonitrile and iso-butanol precipitated dissolved organic residues and reduced water content in GC samples at least by 90%. Results showed that this method could be successfully used for bioethanol production from seaweed.
在本研究中，对海藻用于乙醇生产进行了研究。评估了包括不同温度、酸浓度和孵育时间在内的多种因素组合，以确定合适的糖化条件。选择在120℃下用2.5%（w/v）硫酸处理40分钟的组合来水解海藻生物质，随后进行纯化和发酵以生产乙醇。半乳糖和葡萄糖是海藻水解产物中的主要还原糖，在稀酸水解的最佳条件下，产生了39.42%的还原糖，发酵得到的乙醇浓度为13.27±0.47g/L。评估了一种改进的方法用于气相色谱（GC）分析乙醇含量的样品制备。乙腈和异丁醇的溶剂混合物沉淀了溶解的有机残留物，并使GC样品中的水分含量至少降低了90%。结果表明，该方法可成功用于从海藻生产生物乙醇。
