Medical Sciences Division, Northern Ontario School of Medicine, 955 Oliver Road, Medical School Building, Room 2004, Thunder Bay, Ontario, P7B 5E1, Canada.
Department of Biology, Lakehead University, Thunder Bay, Ontario, Canada.
Muscle Nerve. 2019 Apr;59(4):501-508. doi: 10.1002/mus.26415. Epub 2019 Feb 6.
Muscle precursor cells (MPC) are integral to the maintenance of skeletal muscle and have recently been implicated in playing a role in bone repair. The primary objective of this study was to understand better the role of oxidative stress during the osteogenic differentiation of MPCs.
Muscle precursor cells were treated with various combinations of ascorbic acid (AA), bone morphogenetic protein (BMP)-2, and either a superoxide dismutase analog (4-hydroxy-TEMPO [TEMPOL]) or polyethyleneglycol-conjugated catalase. Muscle precursor cell proliferation and differentiation were determined, and alkaline phosphatase activity was measured as an index of osteogenic differentiation.
After treatment with 200 μM AA, superoxide was increased 1.5-fold, whereas AA in combination with 100 ng/ml BMP-2 did not increase alkaline phosphatase (ALP) activity. When cells were treated with TEMPOL in combination with 100 ng/ml BMP-2 and 200 μM AA, ALP activity significantly increased.
These data suggest that increasing oxidative stress with AA induces sublethal oxidative stress that prevents BMP-2-induced osteogenic differentiation of MPCs. Muscle Nerve 59:501-508, 2019.
肌肉前体细胞（MPC）是维持骨骼肌的重要组成部分，最近有研究表明其在骨修复中发挥作用。本研究的主要目的是更好地了解 MPC 成骨分化过程中氧化应激的作用。
用不同浓度的抗坏血酸（AA）、骨形态发生蛋白 2（BMP-2）和超氧化物歧化酶类似物（4-羟基-TEMPO[TEMPOL]）或聚乙二醇化过氧化氢酶处理肌肉前体细胞。检测肌肉前体细胞的增殖和分化，以碱性磷酸酶活性作为成骨分化的指标。
200 μM AA 处理后，超氧阴离子增加了 1.5 倍，而 AA 与 100ng/ml BMP-2 联合使用并未增加碱性磷酸酶（ALP）活性。当细胞用 TEMPOL 与 100ng/ml BMP-2 和 200 μM AA 联合处理时，ALP 活性显著增加。
这些数据表明，AA 增加氧化应激会诱导亚致死性氧化应激，从而阻止 MPC 中 BMP-2 诱导的成骨分化。肌肉神经 59:501-508, 2019。
