State Key Laboratory of Rice Biology, China National Rice Research Institute, the Chinese Academy of Agricultural Sciences, Hangzhou, 310006, China.
J Integr Plant Biol. 2019 Dec;61(12):1201-1205. doi: 10.1111/jipb.12774. Epub 2019 Mar 6.
High-quality and disease-resistant male sterile lines have great potential for applications in hybrid rice breeding. We introduced specific mutations into the TMS5, Pi21, and Xa13 genes in Pinzhan intermediate breeding material using the CRISPR/Cas9 multiplex genome editing system. We found that the transgene-free homozygous triple tms5/pi21/xa13 mutants obtained in the T generation displayed characteristics of thermosensitive genic male sterility (TGMS) with enhanced resistance to rice blast and bacterial blight. Our study provides a convenient and effective way of converting breeding intermediate material into TGMS lines through multiplex gene editing, which could significantly accelerate the breeding of sterile lines.
高质量和抗病的雄性不育系在杂交水稻育种中有很大的应用潜力。我们使用 CRISPR/Cas9 多重基因组编辑系统，在籼型不育系培矮 64S 中间材料中引入了 TMS5、Pi21 和 Xa13 基因的特定突变。我们发现，在 T 代获得的无转基因纯合三突变体 tms5/pi21/xa13 表现出温敏基因雄性不育（TGMS）的特性，同时增强了对稻瘟病和细菌性条斑病的抗性。我们的研究提供了一种通过多重基因编辑将中间材料转化为 TGMS 系的简便有效方法，这可以显著加速不育系的选育。
